Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 69771..70507 Replicon plasmid p.P39Z1
Accession NZ_CP040352
Organism Klebsiella pneumoniae strain P39

Toxin (Protein)


Gene name tacT Uniprot ID A0A2J5Q928
Locus tag FEZ16_RS25770 Protein ID WP_004098919.1
Coordinates 69771..70253 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A7D3T1D0
Locus tag FEZ16_RS25775 Protein ID WP_004213599.1
Coordinates 70241..70507 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FEZ16_RS25750 (FEZ16_25970) 65125..66471 + 1347 WP_011154555.1 dihydroorotase -
FEZ16_RS25755 (FEZ16_25975) 66535..67557 + 1023 WP_004214536.1 porphobilinogen synthase -
FEZ16_RS25760 (FEZ16_25980) 67732..68127 + 396 Protein_72 IS3 family transposase -
FEZ16_RS25765 (FEZ16_25985) 68123..69350 - 1228 Protein_73 IS3 family transposase -
FEZ16_RS25770 (FEZ16_25990) 69771..70253 - 483 WP_004098919.1 GNAT family N-acetyltransferase Toxin
FEZ16_RS25775 (FEZ16_25995) 70241..70507 - 267 WP_004213599.1 DUF1778 domain-containing protein Antitoxin
FEZ16_RS25780 (FEZ16_26000) 70702..70932 - 231 WP_004213598.1 hypothetical protein -
FEZ16_RS25785 (FEZ16_26005) 70946..71149 - 204 WP_004213596.1 HHA domain-containing protein -
FEZ16_RS25790 (FEZ16_26010) 71210..71704 - 495 WP_004213594.1 hypothetical protein -
FEZ16_RS25795 (FEZ16_26015) 71746..74715 - 2970 WP_094936121.1 Tn3 family transposase -
FEZ16_RS25800 (FEZ16_26020) 74718..75275 - 558 WP_004213590.1 recombinase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - rmpA / iroN / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA / rmpA / pla / pla 1..204102 204102
- inside IScluster/Tn - - 68123..75275 7152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(66-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17335.06 Da        Isoelectric Point: 10.0704

>T125614 WP_004098919.1 NZ_CP040352:c70253-69771 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T125614 NZ_CP040352:c70253-69771 [Klebsiella pneumoniae]
GTGGGACGTATAACCGCGCCCGAGCCGTTATCCAGCGCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCACTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGAAGGGTACGA
AGAAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCTACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGTGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGCGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10151.58 Da        Isoelectric Point: 4.5141

>AT125614 WP_004213599.1 NZ_CP040352:c70507-70241 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRVLIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT125614 NZ_CP040352:c70507-70241 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGTACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGATTCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J5Q928

Antitoxin

Source ID Structure
AlphaFold DB A0A7D3T1D0

References