Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 31924..32834 Replicon plasmid p.P39Z1
Accession NZ_CP040352
Organism Klebsiella pneumoniae strain P39

Toxin (Protein)


Gene name - Uniprot ID A0A663AYG0
Locus tag FEZ16_RS25570 Protein ID WP_004026354.1
Coordinates 32364..32834 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6P1V3Q9
Locus tag FEZ16_RS25565 Protein ID WP_004026357.1
Coordinates 31924..32367 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FEZ16_RS25535 (FEZ16_25760) 26942..27463 + 522 WP_004213635.1 ferric citrate uptake sigma factor FecI -
FEZ16_RS25540 (FEZ16_25765) 27460..28413 + 954 Protein_28 ferric citrate uptake sigma factor regulator FecR -
FEZ16_RS25545 (FEZ16_25770) 28500..30626 + 2127 WP_004213640.1 TonB-dependent Fe(3+) dicitrate receptor FecA -
FEZ16_RS25550 (FEZ16_25775) 30701..30826 + 126 Protein_30 Fe3+-citrate ABC transporter substrate-binding protein -
FEZ16_RS25555 (FEZ16_25780) 31001..31222 + 222 WP_004213643.1 DUF2188 domain-containing protein -
FEZ16_RS25560 (FEZ16_25785) 31320..31823 + 504 Protein_32 DUF4113 domain-containing protein -
FEZ16_RS25565 (FEZ16_25790) 31924..32367 + 444 WP_004026357.1 DUF2384 domain-containing protein Antitoxin
FEZ16_RS25570 (FEZ16_25795) 32364..32834 + 471 WP_004026354.1 RES family NAD+ phosphorylase Toxin
FEZ16_RS25575 (FEZ16_25800) 33459..34156 + 698 WP_223175001.1 IS1-like element IS1A family transposase -
FEZ16_RS25580 (FEZ16_25805) 35542..36234 - 693 WP_237083585.1 DUF1173 family protein -
FEZ16_RS25585 36225..36491 - 267 WP_223175074.1 DUF1173 family protein -
FEZ16_RS25590 (FEZ16_25810) 36669..37163 + 495 WP_004212794.1 thermonuclease family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - rmpA / iroN / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA / rmpA / pla / pla 1..204102 204102
- flank IS/Tn - - 33653..34156 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17515.79 Da        Isoelectric Point: 4.6155

>T125613 WP_004026354.1 NZ_CP040352:32364-32834 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T125613 NZ_CP040352:32364-32834 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
TTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16480.80 Da        Isoelectric Point: 10.2498

>AT125613 WP_004026357.1 NZ_CP040352:31924-32367 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT125613 NZ_CP040352:31924-32367 [Klebsiella pneumoniae]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGCGAATCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCGACCACGTTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAGACAGGAGCGTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AYG0

Antitoxin

Source ID Structure
AlphaFold DB A0A6P1V3Q9

References