Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5194206..5194831 Replicon chromosome
Accession NZ_CP040351
Organism Klebsiella pneumoniae strain P39

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag FEZ16_RS25040 Protein ID WP_002882817.1
Coordinates 5194206..5194589 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag FEZ16_RS25045 Protein ID WP_004150355.1
Coordinates 5194589..5194831 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FEZ16_RS25025 (FEZ16_25225) 5191572..5192474 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
FEZ16_RS25030 (FEZ16_25230) 5192471..5193106 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
FEZ16_RS25035 (FEZ16_25235) 5193103..5194032 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
FEZ16_RS25040 (FEZ16_25240) 5194206..5194589 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
FEZ16_RS25045 (FEZ16_25245) 5194589..5194831 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
FEZ16_RS25050 (FEZ16_25250) 5195036..5195953 + 918 WP_012737090.1 alpha/beta hydrolase -
FEZ16_RS25055 (FEZ16_25255) 5195967..5196908 - 942 WP_012543287.1 fatty acid biosynthesis protein FabY -
FEZ16_RS25060 (FEZ16_25260) 5196953..5197390 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
FEZ16_RS25065 (FEZ16_25265) 5197387..5198247 - 861 WP_004146232.1 virulence factor BrkB family protein -
FEZ16_RS25070 (FEZ16_25270) 5198241..5198840 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T125612 WP_002882817.1 NZ_CP040351:c5194589-5194206 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T125612 NZ_CP040351:c5194589-5194206 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT125612 WP_004150355.1 NZ_CP040351:c5194831-5194589 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT125612 NZ_CP040351:c5194831-5194589 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATACCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References