Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 1872925..1873567 Replicon chromosome
Accession NZ_CP040344
Organism Bacillus albus strain DLOU-Yingkou

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag EQO03_RS09905 Protein ID WP_000635963.1
Coordinates 1873217..1873567 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag EQO03_RS09900 Protein ID WP_000004570.1
Coordinates 1872925..1873212 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQO03_RS09875 1868094..1869056 + 963 WP_048529174.1 UV DNA damage repair endonuclease UvsE -
EQO03_RS09880 1869219..1869767 - 549 WP_098345068.1 rhomboid family intramembrane serine protease -
EQO03_RS09885 1869860..1870219 + 360 WP_048529178.1 holo-ACP synthase -
EQO03_RS09890 1870376..1871326 + 951 WP_002096914.1 outer membrane lipoprotein carrier protein LolA -
EQO03_RS09895 1871445..1872614 + 1170 WP_157409272.1 alanine racemase -
EQO03_RS09900 1872925..1873212 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
EQO03_RS09905 1873217..1873567 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
EQO03_RS09910 1873636..1875804 + 2169 WP_048529180.1 RNA-binding transcriptional accessory protein -
EQO03_RS09915 1875863..1875979 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
EQO03_RS09920 1876175..1876633 + 459 WP_048529183.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T125570 WP_000635963.1 NZ_CP040344:1873217-1873567 [Bacillus albus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T125570 NZ_CP040344:1873217-1873567 [Bacillus albus]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CTAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCATTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT125570 WP_000004570.1 NZ_CP040344:1872925-1873212 [Bacillus albus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT125570 NZ_CP040344:1872925-1873212 [Bacillus albus]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References