125568

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 924285..924927 Replicon chromosome
Accession NZ_CP040342
Organism Bacillus cereus strain DLOU-Weihai

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag EQK03_RS04535 Protein ID WP_000635965.1
Coordinates 924285..924635 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag EQK03_RS04540 Protein ID WP_000004570.1
Coordinates 924640..924927 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQK03_RS04520 921221..921679 - 459 WP_000344237.1 SprT family protein -
EQK03_RS04525 921875..921991 + 117 WP_001143642.1 cortex morphogenetic protein CmpA -
EQK03_RS04530 922049..924217 - 2169 WP_000426236.1 RNA-binding transcriptional accessory protein -
EQK03_RS04535 924285..924635 - 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
EQK03_RS04540 924640..924927 - 288 WP_000004570.1 antitoxin EndoAI Antitoxin
EQK03_RS04545 925236..926405 - 1170 WP_000390615.1 alanine racemase -
EQK03_RS04550 926523..927473 - 951 WP_002004297.1 outer membrane lipoprotein carrier protein LolA -
EQK03_RS04555 927630..927989 - 360 WP_000583415.1 holo-ACP synthase -
EQK03_RS04560 928083..928654 + 572 Protein_847 rhomboid family intramembrane serine protease -
EQK03_RS04565 928647..929609 - 963 WP_000961166.1 UV DNA damage repair endonuclease UvsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T125568 WP_000635965.1 NZ_CP040342:c924635-924285 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T125568 NZ_CP040342:c924635-924285 [Bacillus cereus]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTATGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTGGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT125568 WP_000004570.1 NZ_CP040342:c924927-924640 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT125568 NZ_CP040342:c924927-924640 [Bacillus cereus]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAATTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References