Detailed information of TA system TA125567

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 3082608..3083586
• Replicon: chromosome
• Accession: NZ_CP040340
• Organism: Bacillus cereus strain DLOU-Tangshan

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: W8Y388
• Locus tag: EUC38_RS15630
• Protein ID: WP_000624977.1
• Coordinates: 3082608..3083345 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: M1PKC2
• Locus tag: EUC38_RS15635
• Protein ID: WP_000237819.1
• Coordinates: 3083458..3083586 (+)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EUC38_RS15605	3078221..3078628	+	408	WP_000063587.1	VOC family protein	-
EUC38_RS15610	3078652..3079032	+	381	WP_002025805.1	hypothetical protein	-
EUC38_RS15615	3079190..3080875	-	1686	WP_088054504.1	alpha-keto acid decarboxylase family protein	-
EUC38_RS15620	3080983..3081465	+	483	WP_000191888.1	MarR family winged helix-turn-helix transcriptional regulator	-
EUC38_RS15625	3081632..3082369	+	738	WP_000594152.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
EUC38_RS15630	3082608..3083345	+	738	WP_000624977.1	stage II sporulation protein SA	Toxin
EUC38_RS15635	3083458..3083586	+	129	WP_000237819.1	hypothetical protein	Antitoxin
EUC38_RS15640	3083659..3083835	+	177	WP_000852629.1	stage II sporulation protein SB	-
EUC38_RS15645	3083855..3084244	-	390	Protein_3015	YxeA family protein	-
EUC38_RS15650	3084456..3085925	+	1470	WP_065382524.1	beta-Ala-His dipeptidase	-
EUC38_RS15655	3086171..3086980	+	810	WP_000678370.1	C39 family peptidase	-
EUC38_RS15660	3087006..3087608	+	603	WP_000517271.1	hypothetical protein	-
EUC38_RS15665	3087852..3088103	-	252	WP_000827560.1	response regulator transcription factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3050461..3088695	38234

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28307.03 Da
• Isoelectric Point: 8.2998

>T125567 WP_000624977.1 NZ_CP040340:3082608-3083345 [Bacillus cereus]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T125567 NZ_CP040340:3082608-3083345 [Bacillus cereus]
ATGATCTCTAACATTCGAATCGGCTTATTTGTTTTAGCGATTGTCTTTGTAGTCCTTGTTTTCTTTTATTGGAAAAATGA
GGAGTTGTACGAGGAGAAAAAGCAACGTATTAGAAAGACTTGGTATGGTTTGTTTATCATATCTGTCACCGTGTATTTCA
TGATAAAAGGAATTGATTTAACCCTCTGGAAAAATCTTTTAATGTTTACTGCAATGGTTATTTTTGTTGATATTGCGTTC
ATTTTAACGCCTAATATTTCAGAAATATGGGGTGCGAAATTTAGCGATATTGGCAAGACAGTTCAATCAATAAAACGATC
ATTAATTGCATCTAAAGCGAGAGGGGAAATATACACGACAATTATTCAAAATGTTAATCCAGCAGTATTCGGTACGATGG
AATGGCATACGGAAGAGGAATATACAAAGAGCTTAAATGCATTTTTAGATTCATATGGGGAAAAGATTGGCGCGAAAATT
GTTGTTTTTGAAGCCGCGAAAGAGTTAAATACAAACTTTCGTGGTATTCGCTCGCAATTTAGTATTATCGTTCCGTTAGA
ACATATCGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTAGAAAATGTCGGGATTATACCAGCAAAAATAGTAAGTGACG
TTTTCATTGTTATTGATGGAAAGAAAAATAACCTGCAAGATCGGGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 4953.36 Da
• Isoelectric Point: 3.9798

>AT125567 WP_000237819.1 NZ_CP040340:3083458-3083586 [Bacillus cereus]
VVKDYDNDFFKEGNEDTTDFSLIKGDTLQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT125567 NZ_CP040340:3083458-3083586 [Bacillus cereus]
GTGGTAAAAGATTATGACAATGATTTTTTTAAAGAAGGTAACGAAGATACAACAGATTTCTCTTTAATAAAAGGGGATAC
GTTGCAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATCATAA

Structures

Toxin

• AlphaFold DB: W8Y388

Antitoxin

• AlphaFold DB: M1PKC2