Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 4878032..4878674 Replicon chromosome
Accession NZ_CP040336
Organism Bacillus luti strain FJ

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag EUC40_RS24550 Protein ID WP_000635965.1
Coordinates 4878032..4878382 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag EUC40_RS24555 Protein ID WP_000004570.1
Coordinates 4878387..4878674 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EUC40_RS24535 4874955..4875413 - 459 WP_071712635.1 SprT family protein -
EUC40_RS24540 4875621..4875734 + 114 WP_001143641.1 cortex morphogenetic protein CmpA -
EUC40_RS24545 4875796..4877964 - 2169 WP_156576813.1 RNA-binding transcriptional accessory protein -
EUC40_RS24550 4878032..4878382 - 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
EUC40_RS24555 4878387..4878674 - 288 WP_000004570.1 antitoxin EndoAI Antitoxin
EUC40_RS24560 4878975..4880144 - 1170 WP_071712633.1 alanine racemase -
EUC40_RS24565 4880263..4881213 - 951 WP_071712636.1 outer membrane lipoprotein carrier protein LolA -
EUC40_RS24570 4881370..4881729 - 360 WP_000635046.1 holo-ACP synthase -
EUC40_RS24575 4881822..4882394 + 573 WP_156576814.1 rhomboid family intramembrane serine protease -
EUC40_RS24580 4882387..4883349 - 963 WP_156576815.1 UV DNA damage repair endonuclease UvsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T125565 WP_000635965.1 NZ_CP040336:c4878382-4878032 [Bacillus luti]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T125565 NZ_CP040336:c4878382-4878032 [Bacillus luti]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTGGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT125565 WP_000004570.1 NZ_CP040336:c4878674-4878387 [Bacillus luti]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT125565 NZ_CP040336:c4878674-4878387 [Bacillus luti]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References