Detailed information of TA system TA125564

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 3942332..3943310
• Replicon: chromosome
• Accession: NZ_CP040334
• Organism: Bacillus cereus strain DLOU-Changhai

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: M1Q940
• Locus tag: EUC39_RS19735
• Protein ID: WP_000624982.1
• Coordinates: 3942573..3943310 (-)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: W8YZL5
• Locus tag: EUC39_RS19730
• Protein ID: WP_000237818.1
• Coordinates: 3942332..3942460 (-)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EUC39_RS19705	3938313..3938915	-	603	WP_153592980.1	hypothetical protein	-
EUC39_RS19710	3938941..3939750	-	810	WP_000678370.1	C39 family peptidase	-
EUC39_RS19715	3939996..3941465	-	1470	WP_157418095.1	beta-Ala-His dipeptidase	-
EUC39_RS19720	3941677..3942066	+	390	WP_000713869.1	YxeA family protein	-
EUC39_RS19725	3942083..3942259	-	177	WP_000852629.1	stage II sporulation protein SB	-
EUC39_RS19730	3942332..3942460	-	129	WP_000237818.1	hypothetical protein	Antitoxin
EUC39_RS19735	3942573..3943310	-	738	WP_000624982.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
EUC39_RS19740	3943549..3944286	-	738	WP_000594152.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
EUC39_RS19745	3944453..3944935	-	483	WP_000191889.1	MarR family winged helix-turn-helix transcriptional regulator	-
EUC39_RS19750	3945043..3946728	+	1686	WP_189340001.1	alpha-keto acid decarboxylase family protein	-
EUC39_RS19755	3946886..3947668	-	783	Protein_3819	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28323.03 Da
• Isoelectric Point: 8.2998

>T125564 WP_000624982.1 NZ_CP040334:c3943310-3942573 [Bacillus cereus]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPSVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T125564 NZ_CP040334:c3943310-3942573 [Bacillus cereus]
ATGATCTCTAACATTCGAATTGGCTTATTTGTTTTAGCGATTGTCTTTGTAGTCCTTGTTTTCTTTTATTGGAAAAATGA
GGAGTTGTACGAGGAGAAAAAGCAACGTATTAGAAAGACTTGGTATGGTTTGTTTATCATATCTGTCACCGTGTATTTCA
TGATAAAAGGAATTGATTTAACCCTCTGGAAAAATCTTTTAATGTTTACTGCAATGGTTATTTTTGTTGATATTGCGTTC
ATTTTAACACCTAATATTTCAGAAATATGGGGTGCGAAATTTAGTGATATTGGCAAGACTGTTCAATCAATAAAACGATC
ATTAATTGCATCTAAAGCGAGAGGAGAAATATACACGACAATTATTCAAAATGTTAATCCATCAGTATTTGGTACGATGG
AATGGCATACGGAAGAGGAATATACAAAGAGCTTAAATGCATTTTTAGATTCATATGGGGAAAAGATTGGTGCGAAAATT
GTTGTTTTTGAAGCCGCGAAGGAATTAAATACAAACTTTCGTGGTATTCGCTCGCAATTTAGTATTATCGTTCCGTTAGA
ACATATCGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTAGAAAATGTCGGGATTATACCAGCAAAAATAGTAAGTGACG
TTTTCATTGTTATTGATGGAAAGAAAAATAACCTGCAAGATCGGGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 5011.40 Da
• Isoelectric Point: 3.9150

>AT125564 WP_000237818.1 NZ_CP040334:c3942460-3942332 [Bacillus cereus]
VVKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT125564 NZ_CP040334:c3942460-3942332 [Bacillus cereus]
GTGGTAAAAGATTATGACAATGATTTTTTTAAAGAAGATAACGAAGATACAACAGATTTCTCTTTAATAAAAGGGGATAC
GTTGCAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATCATAA

Structures

Toxin

• AlphaFold DB: M1Q940

Antitoxin

• AlphaFold DB: W8YZL5