125563

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 725234..725876 Replicon chromosome
Accession NZ_CP040334
Organism Bacillus cereus strain DLOU-Changhai

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag EUC39_RS03455 Protein ID WP_000635965.1
Coordinates 725234..725584 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag EUC39_RS03460 Protein ID WP_000004570.1
Coordinates 725589..725876 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EUC39_RS03440 722168..722626 - 459 WP_000503730.1 SprT family protein -
EUC39_RS03445 722822..722938 + 117 WP_001143640.1 cortex morphogenetic protein CmpA -
EUC39_RS03450 722997..725165 - 2169 WP_000426207.1 RNA-binding transcriptional accessory protein -
EUC39_RS03455 725234..725584 - 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
EUC39_RS03460 725589..725876 - 288 WP_000004570.1 antitoxin EndoAI Antitoxin
EUC39_RS03465 726185..727354 - 1170 WP_000390593.1 alanine racemase -
EUC39_RS03470 727472..728422 - 951 WP_002094231.1 outer membrane lipoprotein carrier protein LolA -
EUC39_RS03475 728579..728938 - 360 WP_053565408.1 holo-ACP synthase -
EUC39_RS03480 729032..729604 + 573 WP_000908526.1 rhomboid family intramembrane serine protease -
EUC39_RS03485 729597..730559 - 963 WP_157417186.1 UV DNA damage repair endonuclease UvsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T125563 WP_000635965.1 NZ_CP040334:c725584-725234 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T125563 NZ_CP040334:c725584-725234 [Bacillus cereus]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTATGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTAGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT125563 WP_000004570.1 NZ_CP040334:c725876-725589 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT125563 NZ_CP040334:c725876-725589 [Bacillus cereus]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAATTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTATATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCG
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References