Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 5082997..5083628 Replicon chromosome
Accession NZ_CP040316
Organism Escherichia coli strain CV261

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag FEL12_RS25210 Protein ID WP_001259385.1
Coordinates 5083353..5083628 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag FEL12_RS25205 Protein ID WP_000593555.1
Coordinates 5082997..5083356 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FEL12_RS25175 5078109..5078582 + 474 WP_001161553.1 PTS sugar transporter subunit IIA -
FEL12_RS25180 5078579..5078860 + 282 WP_000084025.1 PTS sugar transporter subunit IIB -
FEL12_RS25185 5078937..5080295 + 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
FEL12_RS25190 5080288..5081796 + 1509 WP_001301773.1 carbohydrate kinase -
FEL12_RS25195 5081786..5082055 + 270 WP_000933060.1 HPr family phosphocarrier protein -
FEL12_RS25200 5082087..5082947 + 861 WP_001131788.1 class II aldolase -
FEL12_RS25205 5082997..5083356 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
FEL12_RS25210 5083353..5083628 - 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
FEL12_RS25215 5083701..5084825 - 1125 WP_001301659.1 ABC transporter permease -
FEL12_RS25220 5084825..5087560 - 2736 WP_000149107.1 ribosome-associated ATPase/putative transporter RbbA -
FEL12_RS25225 5087557..5088624 - 1068 WP_000361458.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T125554 WP_001259385.1 NZ_CP040316:c5083628-5083353 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T125554 NZ_CP040316:c5083628-5083353 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT125554 WP_000593555.1 NZ_CP040316:c5083356-5082997 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT125554 NZ_CP040316:c5083356-5082997 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References