Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 5101298..5101929 Replicon chromosome
Accession NZ_CP040314
Organism Escherichia coli strain MA11

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag FEL10_RS25310 Protein ID WP_001259385.1
Coordinates 5101654..5101929 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag FEL10_RS25305 Protein ID WP_000593555.1
Coordinates 5101298..5101657 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FEL10_RS25275 5096410..5096883 + 474 WP_001161553.1 PTS sugar transporter subunit IIA -
FEL10_RS25280 5096880..5097161 + 282 WP_000084025.1 PTS sugar transporter subunit IIB -
FEL10_RS25285 5097238..5098596 + 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
FEL10_RS25290 5098589..5100097 + 1509 WP_001301773.1 carbohydrate kinase -
FEL10_RS25295 5100087..5100356 + 270 WP_000933060.1 HPr family phosphocarrier protein -
FEL10_RS25300 5100388..5101248 + 861 WP_001131788.1 class II aldolase -
FEL10_RS25305 5101298..5101657 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
FEL10_RS25310 5101654..5101929 - 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
FEL10_RS25315 5102002..5103126 - 1125 WP_001301659.1 ABC transporter permease -
FEL10_RS25320 5103126..5105861 - 2736 WP_000149107.1 ribosome-associated ATPase/putative transporter RbbA -
FEL10_RS25325 5105858..5106925 - 1068 WP_000361458.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T125512 WP_001259385.1 NZ_CP040314:c5101929-5101654 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T125512 NZ_CP040314:c5101929-5101654 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT125512 WP_000593555.1 NZ_CP040314:c5101657-5101298 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT125512 NZ_CP040314:c5101657-5101298 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References