Detailed information of TA system TA125496

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 2934070..2934541
• Replicon: chromosome
• Accession: NZ_CP040314
• Organism: Escherichia coli strain MA11

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: FEL10_RS14400
• Protein ID: WP_001303511.1
• Coordinates: 2934263..2934541 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: FEL10_RS14395
• Protein ID: WP_001302048.1
• Coordinates: 2934070..2934261 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FEL10_RS14355	2929277..2929861	-	585	WP_000206793.1	DUF551 domain-containing protein	-
FEL10_RS14360	2929918..2930313	-	396	WP_001118159.1	DUF977 family protein	-
FEL10_RS14365	2930329..2931098	-	770	Protein_2795	DUF1627 domain-containing protein	-
FEL10_RS14370	2931124..2931864	-	741	WP_000788936.1	ATP-binding protein	-
FEL10_RS14375	2931871..2932833	-	963	WP_000095670.1	helix-turn-helix domain-containing protein	-
FEL10_RS14380	2932856..2933281	-	426	WP_000693943.1	Rha family transcriptional regulator	-
FEL10_RS14385	2933278..2933580	-	303	WP_000172738.1	transcriptional regulator	-
FEL10_RS14390	2933678..2934049	+	372	WP_001169686.1	hypothetical protein	-
FEL10_RS14395	2934070..2934261	+	192	WP_001302048.1	hypothetical protein	Antitoxin
FEL10_RS14400	2934263..2934541	+	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FEL10_RS14405	2934894..2935193	+	300	WP_001240334.1	hypothetical protein	-
FEL10_RS14410	2935265..2935483	+	219	WP_001171930.1	hypothetical protein	-
FEL10_RS14420	2936052..2936240	+	189	WP_000449192.1	cell division inhibition protein DicB	-
FEL10_RS14425	2936237..2936428	+	192	WP_001090200.1	DUF1482 family protein	-
FEL10_RS14430	2936521..2938992	+	2472	WP_000048458.1	exonuclease	-
FEL10_RS14435	2939065..2939316	+	252	WP_000005552.1	excisionase family protein	-
FEL10_RS14440	2939351..2939535	+	185	Protein_2809	DUF3596 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleC	2849094..2939506	90412

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T125496 WP_001303511.1 NZ_CP040314:2934263-2934541 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T125496 NZ_CP040314:2934263-2934541 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT125496 WP_001302048.1 NZ_CP040314:2934070-2934261 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT125496 NZ_CP040314:2934070-2934261 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0