Detailed information of TA system TA125475

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 559456..560051
• Replicon: chromosome
• Accession: NZ_CP040314
• Organism: Escherichia coli strain MA11

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: U9Y4M4
• Locus tag: FEL10_RS02655
• Protein ID: WP_000239579.1
• Coordinates: 559701..560051 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: Q8XCF3
• Locus tag: FEL10_RS02650
• Protein ID: WP_001223210.1
• Coordinates: 559456..559707 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FEL10_RS02640	555121..558900	+	3780	WP_000060932.1	autotransporter assembly complex protein TamB	-
FEL10_RS02645	558903..559244	+	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
FEL10_RS02650	559456..559707	+	252	WP_001223210.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
FEL10_RS02655	559701..560051	+	351	WP_000239579.1	endoribonuclease toxin ChpB	Toxin
FEL10_RS02660	560131..560661	-	531	WP_000055075.1	inorganic diphosphatase	-
FEL10_RS02665	560971..561927	+	957	WP_000265942.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
FEL10_RS02670	562067..563569	+	1503	WP_000205805.1	sugar ABC transporter ATP-binding protein	-
FEL10_RS02675	563583..564605	+	1023	WP_001301928.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12511.45 Da
• Isoelectric Point: 5.6219

>T125475 WP_000239579.1 NZ_CP040314:559701-560051 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T125475 NZ_CP040314:559701-560051 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9299.64 Da
• Isoelectric Point: 4.4201

>AT125475 WP_001223210.1 NZ_CP040314:559456-559707 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEVQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT125475 NZ_CP040314:559456-559707 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGTGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y8A8