Detailed information of TA system TA125455

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 2932054..2932525
• Replicon: chromosome
• Accession: NZ_CP040313
• Organism: Escherichia coli strain M7638

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: FEL02_RS14415
• Protein ID: WP_001303511.1
• Coordinates: 2932247..2932525 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: FEL02_RS14410
• Protein ID: WP_001302048.1
• Coordinates: 2932054..2932245 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FEL02_RS14370	2927261..2927845	-	585	WP_000206793.1	DUF551 domain-containing protein	-
FEL02_RS14375	2927902..2928297	-	396	WP_001118159.1	DUF977 family protein	-
FEL02_RS14380	2928313..2929082	-	770	Protein_2796	DUF1627 domain-containing protein	-
FEL02_RS14385	2929108..2929848	-	741	WP_000788936.1	ATP-binding protein	-
FEL02_RS14390	2929855..2930817	-	963	WP_000095670.1	helix-turn-helix domain-containing protein	-
FEL02_RS14395	2930840..2931265	-	426	WP_000693943.1	Rha family transcriptional regulator	-
FEL02_RS14400	2931262..2931564	-	303	WP_000172738.1	transcriptional regulator	-
FEL02_RS14405	2931662..2932033	+	372	WP_001169686.1	hypothetical protein	-
FEL02_RS14410	2932054..2932245	+	192	WP_001302048.1	hypothetical protein	Antitoxin
FEL02_RS14415	2932247..2932525	+	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FEL02_RS14420	2932878..2933177	+	300	WP_001240334.1	hypothetical protein	-
FEL02_RS14425	2933249..2933467	+	219	WP_001171930.1	hypothetical protein	-
FEL02_RS14435	2934036..2934224	+	189	WP_000449192.1	cell division inhibition protein DicB	-
FEL02_RS14440	2934221..2934412	+	192	WP_001090200.1	DUF1482 family protein	-
FEL02_RS14445	2934505..2936976	+	2472	WP_000048458.1	exonuclease	-
FEL02_RS14450	2937049..2937300	+	252	WP_000005552.1	excisionase family protein	-
FEL02_RS14455	2937335..2937519	+	185	Protein_2810	DUF3596 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleA/espI	2890830..2947405	56575
	-	inside	Prophage	-	espM1 / nleA/espI	2830493..2947405	116912

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T125455 WP_001303511.1 NZ_CP040313:2932247-2932525 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T125455 NZ_CP040313:2932247-2932525 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT125455 WP_001302048.1 NZ_CP040313:2932054-2932245 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT125455 NZ_CP040313:2932054-2932245 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0