Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1323780..1324398 Replicon chromosome
Accession NZ_CP040313
Organism Escherichia coli strain M7638

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag FEL02_RS06175 Protein ID WP_001291435.1
Coordinates 1323780..1323998 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag FEL02_RS06180 Protein ID WP_000344800.1
Coordinates 1324024..1324398 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FEL02_RS06140 1319067..1319639 + 573 WP_000779831.1 YbaY family lipoprotein -
FEL02_RS06145 1319672..1319983 - 312 WP_000409911.1 MGMT family protein -
FEL02_RS06155 1320362..1320715 + 354 WP_000878140.1 DUF1428 domain-containing protein -
FEL02_RS06160 1320757..1322307 - 1551 WP_001302952.1 cyclic-guanylate-specific phosphodiesterase PdeB -
FEL02_RS06165 1322471..1322941 - 471 WP_000136192.1 YlaC family protein -
FEL02_RS06170 1323057..1323608 - 552 WP_000102553.1 maltose O-acetyltransferase -
FEL02_RS06175 1323780..1323998 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
FEL02_RS06180 1324024..1324398 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
FEL02_RS06185 1324944..1328093 - 3150 WP_001132452.1 multidrug efflux RND transporter permease subunit -
FEL02_RS06190 1328116..1329309 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T125444 WP_001291435.1 NZ_CP040313:c1323998-1323780 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T125444 NZ_CP040313:c1323998-1323780 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT125444 WP_000344800.1 NZ_CP040313:c1324398-1324024 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT125444 NZ_CP040313:c1324398-1324024 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACTCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References