Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 1769..2328 Replicon plasmid pO157
Accession NZ_CP040312
Organism Escherichia coli strain F3398

Toxin (Protein)


Gene name relE Uniprot ID O82928
Locus tag FEL04_RS26305 Protein ID WP_000421248.1
Coordinates 2053..2328 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID C1J8K5
Locus tag FEL04_RS26300 Protein ID WP_001178089.1
Coordinates 1769..2053 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FEL04_RS26295 1..858 + 858 WP_000130945.1 incFII family plasmid replication initiator RepA -
FEL04_RS26300 1769..2053 + 285 WP_001178089.1 ribbon-helix-helix domain-containing protein Antitoxin
FEL04_RS26305 2053..2328 + 276 WP_000421248.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FEL04_RS26310 2423..2629 + 207 WP_001105064.1 hypothetical protein -
FEL04_RS26315 2729..2944 - 216 Protein_5 transposase -
FEL04_RS26320 2988..3554 - 567 Protein_6 IS3-like element IS600 family transposase -
FEL04_RS26325 3554..3970 - 417 Protein_7 IS91 family transposase -
FEL04_RS26330 3970..4155 - 186 WP_001302179.1 hypothetical protein -
FEL04_RS26335 4332..6542 + 2211 WP_000592771.1 catalase/peroxidase KatP -
FEL04_RS26340 6586..6975 + 390 WP_001172748.1 cytochrome b562 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - espP / stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / toxB 1..92909 92909


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10735.42 Da        Isoelectric Point: 9.9324

>T125433 WP_000421248.1 NZ_CP040312:2053-2328 [Escherichia coli]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T125433 NZ_CP040312:2053-2328 [Escherichia coli]
ATGGAACTGAAGTGGAGCAGTAAAGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10474.89 Da        Isoelectric Point: 5.6558

>AT125433 WP_001178089.1 NZ_CP040312:1769-2053 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Download         Length: 285 bp

>AT125433 NZ_CP040312:1769-2053 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2Z6FAZ0

Antitoxin

Source ID Structure
AlphaFold DB A0A731IHA8

References