Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 5022085..5022716 Replicon chromosome
Accession NZ_CP040311
Organism Escherichia coli strain F3398

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag FEL04_RS24655 Protein ID WP_001259385.1
Coordinates 5022441..5022716 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag FEL04_RS24650 Protein ID WP_000593555.1
Coordinates 5022085..5022444 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FEL04_RS24620 5017197..5017670 + 474 WP_001161553.1 PTS sugar transporter subunit IIA -
FEL04_RS24625 5017667..5017948 + 282 WP_000084025.1 PTS sugar transporter subunit IIB -
FEL04_RS24630 5018025..5019383 + 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
FEL04_RS24635 5019376..5020884 + 1509 WP_001301773.1 carbohydrate kinase -
FEL04_RS24640 5020874..5021143 + 270 WP_000933060.1 HPr family phosphocarrier protein -
FEL04_RS24645 5021175..5022035 + 861 WP_001131788.1 class II aldolase -
FEL04_RS24650 5022085..5022444 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
FEL04_RS24655 5022441..5022716 - 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
FEL04_RS24660 5022789..5023913 - 1125 WP_001301659.1 ABC transporter permease -
FEL04_RS24665 5023913..5026648 - 2736 WP_000149107.1 ribosome-associated ATPase/putative transporter RbbA -
FEL04_RS24670 5026645..5027712 - 1068 WP_000361458.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T125430 WP_001259385.1 NZ_CP040311:c5022716-5022441 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T125430 NZ_CP040311:c5022716-5022441 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT125430 WP_000593555.1 NZ_CP040311:c5022444-5022085 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT125430 NZ_CP040311:c5022444-5022085 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References