Detailed information of TA system TA125404

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 1922355..1922833
• Replicon: chromosome
• Accession: NZ_CP040311
• Organism: Escherichia coli strain F3398

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A891SFN9
• Locus tag: FEL04_RS08805
• Protein ID: WP_001303876.1
• Coordinates: 1922355..1922642 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XD67
• Locus tag: FEL04_RS08810
• Protein ID: WP_000536233.1
• Coordinates: 1922642..1922833 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FEL04_RS08775	1917504..1919975	-	2472	WP_000034474.1	exonuclease	-
FEL04_RS08780	1920069..1920260	-	192	WP_001098307.1	DUF1482 family protein	-
FEL04_RS08785	1920257..1920445	-	189	WP_000413705.1	cell division inhibition protein DicB	-
FEL04_RS08790	1921019..1921204	+	186	WP_001133046.1	hypothetical protein	-
FEL04_RS08795	1921391..1921780	-	390	WP_000394511.1	hypothetical protein	-
FEL04_RS08800	1921922..1922077	-	156	WP_000379575.1	DUF1391 family protein	-
FEL04_RS08805	1922355..1922642	-	288	WP_001303876.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FEL04_RS08810	1922642..1922833	-	192	WP_000536233.1	hypothetical protein	Antitoxin
FEL04_RS08815	1922861..1923262	-	402	WP_000986592.1	helix-turn-helix domain-containing protein	-
FEL04_RS08820	1923371..1923643	+	273	WP_000887453.1	helix-turn-helix domain-containing protein	-
FEL04_RS08825	1923627..1924052	+	426	WP_000693855.1	Rha family transcriptional regulator	-
FEL04_RS08830	1924259..1924741	-	483	WP_153023042.1	hypothetical protein	-
FEL04_RS08840	1926106..1927191	+	1086	WP_072141648.1	DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1908855..1925612	16757

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10978.84 Da
• Isoelectric Point: 10.1360

>T125404 WP_001303876.1 NZ_CP040311:c1922642-1922355 [Escherichia coli]
MLPILWLPSARDDLRQIITYIAKENPPAARRLKIRIETSVLPLSEHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIE
IVSVTHSRRQFPFSI

Nucleotide

• Length: 288 bp

>T125404 NZ_CP040311:c1922642-1922355 [Escherichia coli]
ATGTTACCCATTTTATGGCTACCGTCTGCTCGCGATGATTTGCGCCAGATCATAACTTACATCGCCAAGGAGAACCCACC
GGCAGCACGTAGACTAAAAATACGCATTGAAACATCGGTATTACCTCTATCTGAGCATCCGTACTTATATCCACCAAGCG
AACGGGTTTCTGGATTGAGAGAGATCGTGACCCACCCTAACTACATAATCCTGTACAGAGTAGCTGCTTCAAGCATTGAG
ATTGTAAGCGTGACACATTCTCGGCGACAATTTCCCTTCTCTATCTGA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7025.80 Da
• Isoelectric Point: 4.0270

>AT125404 WP_000536233.1 NZ_CP040311:c1922833-1922642 [Escherichia coli]
MGTALSPIVSEFETTEQENSYNEWLRTKVTSSLADTRPAIPHDEVMAEMENLIAQIAVTNKSE

Nucleotide

• Length: 192 bp

>AT125404 NZ_CP040311:c1922833-1922642 [Escherichia coli]
ATGGGTACAGCCCTTTCTCCGATAGTTTCAGAATTCGAAACTACCGAACAAGAAAACAGTTACAACGAATGGTTGCGCAC
TAAAGTAACGTCAAGCCTTGCAGACACTCGCCCCGCAATTCCACATGACGAGGTAATGGCTGAAATGGAAAATCTTATTG
CTCAAATTGCTGTAACTAACAAGAGCGAGTAA