Detailed information of TA system TA125387

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 4999542..5000342
• Replicon: chromosome
• Accession: NZ_CP040309
• Organism: Escherichia coli strain 21B8

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: A0A891SNC1
• Locus tag: FEK99_RS24690
• Protein ID: WP_000342448.1
• Coordinates: 4999542..5000069 (-)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: F4NNI1
• Locus tag: FEK99_RS24695
• Protein ID: WP_001277108.1
• Coordinates: 5000076..5000342 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FEK99_RS24665	4994617..4995384	-	768	WP_000082101.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
FEK99_RS24670	4995381..4996658	-	1278	WP_000803797.1	branched chain amino acid ABC transporter permease LivM	-
FEK99_RS24675	4996655..4997581	-	927	WP_001295111.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
FEK99_RS24680	4997629..4998738	-	1110	WP_001301528.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
FEK99_RS24685	4999162..4999545	+	384	WP_000778769.1	aspartate 1-decarboxylase autocleavage activator PanM	-
FEK99_RS24690	4999542..5000069	-	528	WP_000342448.1	GNAT family N-acetyltransferase	Toxin
FEK99_RS24695	5000076..5000342	-	267	WP_001277108.1	DUF1778 domain-containing protein	Antitoxin
FEK99_RS24700	5000492..5001595	-	1104	WP_001021996.1	branched chain amino acid ABC transporter substrate-binding protein LivJ	-
FEK99_RS24705	5001866..5002720	-	855	WP_000130217.1	RNA polymerase sigma factor RpoH	-
FEK99_RS24710	5002965..5004023	-	1059	WP_001042003.1	permease-like cell division protein FtsX	-
FEK99_RS24715	5004016..5004684	-	669	WP_000617723.1	cell division ATP-binding protein FtsE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (88-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 19692.68 Da
• Isoelectric Point: 7.3232

>T125387 WP_000342448.1 NZ_CP040309:c5000069-4999542 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Nucleotide

• Length: 528 bp

>T125387 NZ_CP040309:c5000069-4999542 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATAAATCGCTGGGCTTTATCCCTTTAGTCGGGGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATTGAACTGCTTTTTACACAGAGCGATTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9910.31 Da
• Isoelectric Point: 10.0863

>AT125387 WP_001277108.1 NZ_CP040309:c5000342-5000076 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Nucleotide

• Length: 267 bp

>AT125387 NZ_CP040309:c5000342-5000076 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Structures

Toxin

• PDB: 6GTS

Antitoxin

• AlphaFold DB: A0A829CN24