Detailed information of TA system TA125372

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 2937125..2937596
• Replicon: chromosome
• Accession: NZ_CP040309
• Organism: Escherichia coli strain 21B8

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: FEK99_RS14410
• Protein ID: WP_001303511.1
• Coordinates: 2937318..2937596 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: FEK99_RS14405
• Protein ID: WP_001302048.1
• Coordinates: 2937125..2937316 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FEK99_RS14365	2932332..2932916	-	585	WP_000206793.1	DUF551 domain-containing protein	-
FEK99_RS14370	2932973..2933368	-	396	WP_001118159.1	DUF977 family protein	-
FEK99_RS14375	2933384..2934153	-	770	Protein_2796	DUF1627 domain-containing protein	-
FEK99_RS14380	2934179..2934919	-	741	WP_000788936.1	ATP-binding protein	-
FEK99_RS14385	2934926..2935888	-	963	WP_000095670.1	helix-turn-helix domain-containing protein	-
FEK99_RS14390	2935911..2936336	-	426	WP_000693943.1	Rha family transcriptional regulator	-
FEK99_RS14395	2936333..2936635	-	303	WP_000172738.1	transcriptional regulator	-
FEK99_RS14400	2936733..2937104	+	372	WP_001169686.1	hypothetical protein	-
FEK99_RS14405	2937125..2937316	+	192	WP_001302048.1	hypothetical protein	Antitoxin
FEK99_RS14410	2937318..2937596	+	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FEK99_RS14415	2937949..2938248	+	300	WP_001240334.1	hypothetical protein	-
FEK99_RS14420	2938320..2938538	+	219	WP_001171930.1	hypothetical protein	-
FEK99_RS14430	2939107..2939295	+	189	WP_000449192.1	cell division inhibition protein DicB	-
FEK99_RS14435	2939292..2939483	+	192	WP_001090200.1	DUF1482 family protein	-
FEK99_RS14440	2939576..2942047	+	2472	WP_000048458.1	exonuclease	-
FEK99_RS14445	2942120..2942371	+	252	WP_000005552.1	excisionase family protein	-
FEK99_RS14450	2942406..2942590	+	185	Protein_2810	DUF3596 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7' / espM1 / nleA/espI	2791075..2952476	161401
	-	inside	Prophage	-	nleG7' / espM1 / nleA/espI	2789287..2952476	163189

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T125372 WP_001303511.1 NZ_CP040309:2937318-2937596 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T125372 NZ_CP040309:2937318-2937596 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT125372 WP_001302048.1 NZ_CP040309:2937125-2937316 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT125372 NZ_CP040309:2937125-2937316 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• AlphaFold DB: A0A829CTP0