125367

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2658107..2658745 Replicon chromosome
Accession NZ_CP040309
Organism Escherichia coli strain 21B8

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag FEK99_RS12840 Protein ID WP_000813794.1
Coordinates 2658107..2658283 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag FEK99_RS12845 Protein ID WP_001270286.1
Coordinates 2658329..2658745 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FEK99_RS12820 2653729..2654940 - 1212 WP_071525382.1 BenE family transporter YdcO -
FEK99_RS12825 2654993..2655529 + 537 WP_000429145.1 DNA-binding transcriptional regulator SutR -
FEK99_RS12830 2655602..2657563 + 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
FEK99_RS12835 2657655..2657885 - 231 WP_000494244.1 YncJ family protein -
FEK99_RS12840 2658107..2658283 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
FEK99_RS12845 2658329..2658745 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
FEK99_RS12850 2658824..2660230 + 1407 WP_000760654.1 PLP-dependent aminotransferase family protein -
FEK99_RS12855 2660475..2661620 + 1146 WP_000047432.1 ABC transporter substrate-binding protein -
FEK99_RS12860 2661638..2662651 + 1014 WP_000220402.1 ABC transporter ATP-binding protein -
FEK99_RS12865 2662652..2663593 + 942 WP_001251315.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T125367 WP_000813794.1 NZ_CP040309:2658107-2658283 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T125367 NZ_CP040309:2658107-2658283 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT125367 WP_001270286.1 NZ_CP040309:2658329-2658745 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT125367 NZ_CP040309:2658329-2658745 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAAGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References