Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 5136528..5137159 Replicon chromosome
Accession NZ_CP040307
Organism Escherichia coli strain F1 E4

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag FEL01_RS25565 Protein ID WP_001259385.1
Coordinates 5136884..5137159 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag FEL01_RS25560 Protein ID WP_000593555.1
Coordinates 5136528..5136887 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FEL01_RS25530 5131639..5132113 + 475 Protein_4971 PTS sugar transporter subunit IIA -
FEL01_RS25535 5132110..5132391 + 282 WP_000084025.1 PTS sugar transporter subunit IIB -
FEL01_RS25540 5132468..5133826 + 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
FEL01_RS25545 5133819..5135327 + 1509 WP_001301773.1 carbohydrate kinase -
FEL01_RS25550 5135317..5135586 + 270 WP_000933060.1 HPr family phosphocarrier protein -
FEL01_RS25555 5135618..5136478 + 861 WP_001131788.1 class II aldolase -
FEL01_RS25560 5136528..5136887 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
FEL01_RS25565 5136884..5137159 - 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
FEL01_RS25570 5137232..5138356 - 1125 WP_001301659.1 ABC transporter permease -
FEL01_RS25575 5138356..5141091 - 2736 WP_000149107.1 ribosome-associated ATPase/putative transporter RbbA -
FEL01_RS25580 5141088..5142155 - 1068 WP_000361458.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 5131639..5146015 14376


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T125345 WP_001259385.1 NZ_CP040307:c5137159-5136884 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T125345 NZ_CP040307:c5137159-5136884 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT125345 WP_000593555.1 NZ_CP040307:c5136887-5136528 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT125345 NZ_CP040307:c5136887-5136528 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References