Detailed information of TA system TA125329

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 3030237..3030708
• Replicon: chromosome
• Accession: NZ_CP040307
• Organism: Escherichia coli strain F1 E4

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: FEL01_RS15025
• Protein ID: WP_001303511.1
• Coordinates: 3030430..3030708 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: FEL01_RS15020
• Protein ID: WP_001302048.1
• Coordinates: 3030237..3030428 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FEL01_RS14980	3025444..3026028	-	585	WP_000206793.1	DUF551 domain-containing protein	-
FEL01_RS14985	3026085..3026480	-	396	WP_001118159.1	DUF977 family protein	-
FEL01_RS14990	3026496..3027265	-	770	Protein_2920	DUF1627 domain-containing protein	-
FEL01_RS14995	3027291..3028031	-	741	WP_000788936.1	ATP-binding protein	-
FEL01_RS15000	3028038..3029000	-	963	WP_000095670.1	helix-turn-helix domain-containing protein	-
FEL01_RS15005	3029023..3029448	-	426	WP_000693943.1	Rha family transcriptional regulator	-
FEL01_RS15010	3029445..3029747	-	303	WP_000172738.1	transcriptional regulator	-
FEL01_RS15015	3029845..3030216	+	372	WP_001169686.1	hypothetical protein	-
FEL01_RS15020	3030237..3030428	+	192	WP_001302048.1	hypothetical protein	Antitoxin
FEL01_RS15025	3030430..3030708	+	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FEL01_RS15030	3031061..3031360	+	300	WP_001240334.1	hypothetical protein	-
FEL01_RS15035	3031432..3031650	+	219	WP_001171930.1	hypothetical protein	-
FEL01_RS15045	3032219..3032407	+	189	WP_000449192.1	cell division inhibition protein DicB	-
FEL01_RS15050	3032404..3032595	+	192	WP_001090200.1	DUF1482 family protein	-
FEL01_RS15055	3032688..3035159	+	2472	WP_000048458.1	exonuclease	-
FEL01_RS15060	3035232..3035483	+	252	WP_000005552.1	excisionase family protein	-
FEL01_RS15065	3035518..3035702	+	185	Protein_2934	DUF3596 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2992898..3037869	44971

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T125329 WP_001303511.1 NZ_CP040307:3030430-3030708 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T125329 NZ_CP040307:3030430-3030708 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT125329 WP_001302048.1 NZ_CP040307:3030237-3030428 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT125329 NZ_CP040307:3030237-3030428 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0