Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1323772..1324390 Replicon chromosome
Accession NZ_CP040307
Organism Escherichia coli strain F1 E4

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag FEL01_RS06175 Protein ID WP_001291435.1
Coordinates 1323772..1323990 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag FEL01_RS06180 Protein ID WP_000344800.1
Coordinates 1324016..1324390 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FEL01_RS06140 1319059..1319631 + 573 WP_000779831.1 YbaY family lipoprotein -
FEL01_RS06145 1319664..1319975 - 312 WP_000409911.1 MGMT family protein -
FEL01_RS06155 1320354..1320707 + 354 WP_000878140.1 DUF1428 domain-containing protein -
FEL01_RS06160 1320749..1322299 - 1551 WP_001302952.1 cyclic-guanylate-specific phosphodiesterase PdeB -
FEL01_RS06165 1322463..1322933 - 471 WP_000136192.1 YlaC family protein -
FEL01_RS06170 1323049..1323600 - 552 WP_000102553.1 maltose O-acetyltransferase -
FEL01_RS06175 1323772..1323990 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
FEL01_RS06180 1324016..1324390 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
FEL01_RS06185 1324936..1328085 - 3150 WP_001132452.1 multidrug efflux RND transporter permease subunit -
FEL01_RS06190 1328108..1329301 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T125324 WP_001291435.1 NZ_CP040307:c1323990-1323772 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T125324 NZ_CP040307:c1323990-1323772 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT125324 WP_000344800.1 NZ_CP040307:c1324390-1324016 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT125324 NZ_CP040307:c1324390-1324016 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACTCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References