125265

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 4341751..4342376 Replicon chromosome
Accession NZ_CP040269
Organism Escherichia coli strain 1500

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag EST53_RS21080 Protein ID WP_000911329.1
Coordinates 4341978..4342376 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag EST53_RS21075 Protein ID WP_000450524.1
Coordinates 4341751..4341978 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EST53_RS21050 4337553..4338023 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
EST53_RS21055 4338023..4338595 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
EST53_RS21060 4338741..4339619 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
EST53_RS21065 4339636..4340670 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
EST53_RS21070 4340883..4341596 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
EST53_RS21075 4341751..4341978 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
EST53_RS21080 4341978..4342376 + 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
EST53_RS21085 4342523..4343386 + 864 WP_001267498.1 neutral zinc metallopeptidase -
EST53_RS21090 4343401..4345416 + 2016 WP_053264617.1 tRNA cytosine(34) acetyltransferase TmcA -
EST53_RS21095 4345490..4346188 + 699 WP_000679812.1 esterase -
EST53_RS21100 4346269..4346469 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T125265 WP_000911329.1 NZ_CP040269:4341978-4342376 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T125265 NZ_CP040269:4341978-4342376 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT125265 WP_000450524.1 NZ_CP040269:4341751-4341978 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT125265 NZ_CP040269:4341751-4341978 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References