Detailed information of TA system TA125259

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yeeU/CbtA-CbeA
• Location: 2367493..2368200
• Replicon: chromosome
• Accession: NZ_CP040269
• Organism: Escherichia coli strain 1500

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: EST53_RS11460
• Protein ID: WP_053264534.1
• Coordinates: 2367493..2367834 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A8S7X7W3
• Locus tag: EST53_RS11465
• Protein ID: WP_053264533.1
• Coordinates: 2367865..2368200 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EST53_RS11425	2362900..2363880	+	981	WP_053264536.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
EST53_RS11430	2363888..2364538	-	651	WP_001037963.1	HNH endonuclease	-
EST53_RS23720	2364917..2365102	+	186	WP_000066585.1	hypothetical protein	-
EST53_RS11445	2365140..2366087	-	948	WP_000342497.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
EST53_RS11450	2366080..2366475	-	396	WP_000152741.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	-
EST53_RS11455	2366545..2367378	-	834	WP_053264535.1	DUF4942 domain-containing protein	-
EST53_RS11460	2367493..2367834	-	342	WP_053264534.1	TA system toxin CbtA family protein	Toxin
EST53_RS11465	2367865..2368200	-	336	WP_053264533.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
EST53_RS11470	2368200..2368673	-	474	WP_001296921.1	DNA repair protein RadC	-
EST53_RS11475	2368703..2369521	-	819	WP_053264532.1	DUF945 domain-containing protein	-
EST53_RS11480	2369757..2370710	-	954	WP_053264531.1	hypothetical protein	-
EST53_RS11485	2371303..2371917	+	615	WP_000772910.1	inovirus Gp2 family protein	-
EST53_RS11490	2372035..2372253	+	219	WP_001064742.1	AlpA family phage regulatory protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimC / fimI / fimA / fimE / fimB	2355467..2386429	30962

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-109)

Antitoxin

• Antitoxin CbeA (10-110)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13074.11 Da
• Isoelectric Point: 9.4583

>T125259 WP_053264534.1 NZ_CP040269:c2367834-2367493 [Escherichia coli]
MKIIPATTLRATTSYLSPVVVWQTLLARLLEQHYGLNLNDTPFNNEKVIQEHIDAGITLVDAINFLVEKYELIRIDRKGF
SWQEQTPYLRAVDILRARQATGLLRRCYNTTAR

Nucleotide

• Length: 342 bp

>T125259 NZ_CP040269:c2367834-2367493 [Escherichia coli]
ATGAAAATTATTCCTGCAACGACGCTGCGGGCGACGACGTCATATCTGTCGCCTGTCGTGGTCTGGCAAACATTACTGGC
CCGCCTGCTGGAGCAGCACTACGGGCTGAATCTTAATGACACACCATTCAACAATGAAAAGGTGATTCAGGAACACATAG
ACGCCGGAATCACTCTGGTCGATGCCATCAATTTTCTGGTGGAGAAGTACGAATTGATCCGTATCGACAGGAAAGGATTT
AGCTGGCAGGAACAGACGCCTTATCTTCGTGCGGTCGATATTTTGCGGGCGCGGCAGGCTACAGGTCTGCTGCGCCGCTG
CTATAACACCACGGCACGGTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12593.55 Da
• Isoelectric Point: 8.4816

>AT125259 WP_053264533.1 NZ_CP040269:c2368200-2367865 [Escherichia coli]
MLQKDKVMQQEWGLPCTVTPRFTARLVQERHNLHYLADRAAITGKFSSPDLLHIDQAFPVLMKQLELKLADGELSPRQQR
TVTLYAKGLTCEADTLGSSGYVYIAIYPTQR

Nucleotide

• Length: 336 bp

>AT125259 NZ_CP040269:c2368200-2367865 [Escherichia coli]
ATGCTGCAAAAGGACAAGGTCATGCAACAAGAATGGGGGCTTCCCTGTACCGTAACACCACGCTTCACTGCTCGACTGGT
ACAGGAAAGACATAACCTGCATTACCTGGCTGATCGGGCCGCAATCACCGGGAAATTCAGCTCCCCGGATCTGCTGCATA
TCGACCAGGCTTTTCCGGTGCTGATGAAACAACTGGAGCTGAAACTGGCTGACGGTGAACTCTCCCCCCGCCAACAACGT
ACCGTCACGCTATACGCCAAAGGACTAACCTGCGAAGCCGATACGCTGGGTTCCAGTGGTTACGTCTATATCGCTATTTA
TCCCACTCAACGCTGA