125258

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) sanaTA/AbiEii-DUF6088
Location 2365140..2366475 Replicon chromosome
Accession NZ_CP040269
Organism Escherichia coli strain 1500

Toxin (Protein)


Gene name sanaT Uniprot ID F4VMR9
Locus tag EST53_RS11445 Protein ID WP_000342497.1
Coordinates 2365140..2366087 (-) Length 316 a.a.

Antitoxin (Protein)


Gene name sanaA Uniprot ID E0J2C6
Locus tag EST53_RS11450 Protein ID WP_000152741.1
Coordinates 2366080..2366475 (-) Length 132 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EST53_RS11415 2360993..2361709 + 717 WP_021534608.1 N-acetylneuraminic acid outer membrane channel NanC -
EST53_RS11420 2361729..2362835 + 1107 WP_032217392.1 N-acetylneuraminate epimerase -
EST53_RS11425 2362900..2363880 + 981 WP_053264536.1 9-O-acetyl-N-acetylneuraminic acid deacetylase -
EST53_RS11430 2363888..2364538 - 651 WP_001037963.1 HNH endonuclease -
EST53_RS23720 2364917..2365102 + 186 WP_000066585.1 hypothetical protein -
EST53_RS11445 2365140..2366087 - 948 WP_000342497.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein Toxin
EST53_RS11450 2366080..2366475 - 396 WP_000152741.1 type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein Antitoxin
EST53_RS11455 2366545..2367378 - 834 WP_053264535.1 DUF4942 domain-containing protein -
EST53_RS11460 2367493..2367834 - 342 WP_053264534.1 TA system toxin CbtA family protein -
EST53_RS11465 2367865..2368200 - 336 WP_053264533.1 type IV toxin-antitoxin system YeeU family antitoxin -
EST53_RS11470 2368200..2368673 - 474 WP_001296921.1 DNA repair protein RadC -
EST53_RS11475 2368703..2369521 - 819 WP_053264532.1 DUF945 domain-containing protein -
EST53_RS11480 2369757..2370710 - 954 WP_053264531.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - fimC / fimI / fimA / fimE / fimB 2355467..2386429 30962


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Nucleotidyl transferase AbiEii toxin, Type IV TA system

(38-303)

Antitoxin

Protein of unknown function DUF6088

(7-108)


Sequences

Toxin        


Download         Length: 316 a.a.        Molecular weight: 35588.79 Da        Isoelectric Point: 4.9737

>T125258 WP_000342497.1 NZ_CP040269:c2366087-2365140 [Escherichia coli]
MDDLTTLFSEVADALGIESVAIVEKDHYIVELLQLLKPLVFDTHQLVFAGGTALAKSGVALNRMSEDVDIKLVPKDAFQA
LSRTQRKNTRKEIVQAVSSAIASSGVFNFDDDYPKVTRDEYRYNDIPVRYPQRVVQAPCLRPFIRLELMEAKLLEPSELR
DIHSLVTGITGKAAIVSAFPCATIISTQAEKLVAMLRRTAAVVRDVERFDDASLVRHIYDTNCIVETSHVDMSLLVDFVQ
QTIEQDIQRYGNQYPQFCASPVDELKMGLDELANNPLYQMRYQQFVSPMVYGERKITWDEAYSRFRSLALAILNA

Download         Length: 948 bp

>T125258 NZ_CP040269:c2366087-2365140 [Escherichia coli]
ATGGATGATTTAACCACTCTTTTTTCTGAAGTGGCTGACGCGCTTGGTATTGAGTCTGTCGCAATTGTGGAAAAAGATCA
CTATATCGTTGAGCTTTTGCAATTGCTTAAACCTCTGGTGTTCGATACTCATCAATTGGTGTTTGCCGGAGGAACCGCTC
TGGCAAAATCAGGTGTCGCACTTAATCGCATGTCGGAAGATGTGGATATCAAACTGGTGCCTAAGGATGCATTTCAGGCA
CTTTCCCGCACTCAGCGAAAAAATACCCGTAAAGAAATTGTTCAGGCAGTTTCCAGTGCAATAGCTTCATCGGGTGTGTT
CAATTTTGATGATGATTACCCAAAAGTGACGCGGGATGAATATCGTTATAATGACATACCTGTTCGTTATCCACAGCGAG
TAGTGCAGGCACCTTGTTTGCGTCCTTTTATCCGGCTGGAACTGATGGAAGCGAAGTTACTGGAGCCTTCTGAATTACGG
GATATTCATTCACTGGTAACAGGGATCACGGGAAAAGCGGCTATTGTTTCAGCGTTTCCATGCGCAACTATCATTAGCAC
ACAGGCAGAAAAGCTGGTGGCGATGTTACGTCGTACTGCGGCAGTTGTGCGAGATGTTGAGCGATTTGATGATGCATCGC
TGGTACGTCATATCTACGATACAAACTGTATTGTTGAGACGAGTCATGTTGATATGAGTCTGCTCGTTGATTTTGTTCAA
CAAACTATTGAGCAGGACATTCAGCGTTACGGCAACCAGTACCCGCAGTTTTGCGCGTCTCCAGTTGATGAGCTAAAAAT
GGGACTGGATGAGTTGGCGAACAATCCTCTCTACCAGATGCGCTATCAGCAGTTTGTTTCGCCAATGGTGTATGGAGAGC
GAAAGATCACATGGGATGAGGCTTACTCCAGGTTTAGGAGCTTAGCGCTAGCCATCCTGAATGCTTGA

Antitoxin


Download         Length: 132 a.a.        Molecular weight: 14619.93 Da        Isoelectric Point: 11.6915

>AT125258 WP_000152741.1 NZ_CP040269:c2366475-2366080 [Escherichia coli]
MTIKARIQSRLKRSKRYVFTRDDFKDIAGYDQVGRVLRELVREGQLLKVGYGVYTKARRNGITGKVMPAAPGGSSAVIVE
TLERLKVRYRFAGATQAYNSGTSTQIPASPEIKTPRRFKRVLSVGNSRLNG

Download         Length: 396 bp

>AT125258 NZ_CP040269:c2366475-2366080 [Escherichia coli]
ATGACGATAAAAGCACGCATTCAGTCACGACTGAAACGTTCTAAACGTTACGTATTCACTCGTGACGACTTTAAAGATAT
TGCAGGCTACGATCAGGTTGGTCGGGTATTGCGCGAGCTTGTCAGGGAAGGGCAACTGTTGAAGGTTGGTTATGGCGTGT
ATACCAAAGCACGGCGGAACGGTATTACAGGCAAGGTAATGCCAGCGGCTCCCGGAGGCTCTTCAGCGGTGATTGTCGAA
ACGCTGGAACGCCTGAAAGTACGCTATCGTTTTGCTGGCGCAACGCAGGCATACAATAGCGGTACTTCTACGCAGATCCC
AGCTTCACCCGAGATAAAAACCCCCCGGCGTTTTAAACGCGTATTATCTGTAGGGAACAGCAGATTAAATGGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1L5BAQ6

Antitoxin

Source ID Structure
AlphaFold DB A0A1L4ZXD4

References