Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 1756840..1757677 Replicon chromosome
Accession NZ_CP040269
Organism Escherichia coli strain 1500

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag EST53_RS08565 Protein ID WP_000227784.1
Coordinates 1757135..1757677 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag EST53_RS08560 Protein ID WP_001297137.1
Coordinates 1756840..1757151 (+) Length 104 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EST53_RS08535 1751860..1752807 + 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -
EST53_RS08540 1752829..1754820 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
EST53_RS08545 1754810..1755424 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
EST53_RS08550 1755424..1755753 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
EST53_RS08555 1755765..1756655 + 891 WP_000971336.1 heme o synthase -
EST53_RS08560 1756840..1757151 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
EST53_RS08565 1757135..1757677 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
EST53_RS08570 1757733..1758668 - 936 Protein_1664 sel1 repeat family protein -
EST53_RS08575 1759076..1760440 + 1365 WP_001000978.1 MFS transporter -
EST53_RS08580 1760568..1761059 - 492 WP_001138904.1 nucleotide binding protein YajQ -
EST53_RS08585 1761227..1762138 + 912 WP_000705874.1 2-dehydropantoate 2-reductase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(68-156)

Antitoxin

(14-97)


Sequences


Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T125256 WP_000227784.1 NZ_CP040269:1757135-1757677 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T125256 NZ_CP040269:1757135-1757677 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT125256 WP_001297137.1 NZ_CP040269:1756840-1757151 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT125256 NZ_CP040269:1756840-1757151 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
TGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 7BYY


Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References