Detailed information of TA system TA125244

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 1932..2491
• Replicon: plasmid pEc631_2
• Accession: NZ_CP040265
• Organism: Escherichia coli strain 631

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: EST51_RS26095
• Protein ID: WP_075858635.1
• Coordinates: 2216..2491 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: EST51_RS26090
• Protein ID: WP_075858633.1
• Coordinates: 1932..2216 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EST51_RS26075	1..1023	+	1023	WP_150395047.1	replication initiation protein	-
EST51_RS26090	1932..2216	+	285	WP_075858633.1	ribbon-helix-helix protein, CopG family	Antitoxin
EST51_RS26095	2216..2491	+	276	WP_075858635.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EST51_RS26100	2635..2901	+	267	WP_075858637.1	hypothetical protein	-
EST51_RS26105	2842..3051	-	210	Protein_5	Tn3 family transposase	-
EST51_RS26115	3240..3872	-	633	Protein_6	transposase	-
EST51_RS27120	3962..4093	+	132	Protein_7	helix-turn-helix domain-containing protein	-
EST51_RS27125	4072..4206	-	135	Protein_8	transposase zinc-binding domain-containing protein	-
EST51_RS26125	4244..4387	+	144	WP_190320372.1	hypothetical protein	-
EST51_RS26130	4517..6313	+	1797	WP_075858538.1	AIPR family protein	-
EST51_RS26135	6648..6962	-	315	Protein_11	DUF4158 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..73952	73952

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10766.48 Da
• Isoelectric Point: 9.9324

>T125244 WP_075858635.1 NZ_CP040265:2216-2491 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLTSKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELTGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T125244 NZ_CP040265:2216-2491 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGACCAGTAAACCTGC
TGCCGCCAGAACGGTGCAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTACTGGCCAGACTATTTAT
GTATTGCGTCTGTGGCATACGCGGGAAAACAGATAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10493.96 Da
• Isoelectric Point: 6.4613

>AT125244 WP_075858633.1 NZ_CP040265:1932-2216 [Escherichia coli]
MQMKNNSAQATKVITAHVPLLMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEAMDDVTSGQVINHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT125244 NZ_CP040265:1932-2216 [Escherichia coli]
ATGCAAATGAAAAACAATAGTGCACAAGCAACAAAAGTAATTACCGCACATGTGCCATTACTTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGGCTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCATGGACGATGTAACATCCGGACAGGTTATCAACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA