Detailed information of TA system TA125243

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 1822..2357
• Replicon: plasmid pEc631_1
• Accession: NZ_CP040264
• Organism: Escherichia coli strain 631

Toxin (Protein)

• Gene name: relE
• Uniprot ID: T2HS21
• Locus tag: EST51_RS25475
• Protein ID: WP_000222775.1
• Coordinates: 2070..2357 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: T2HSA2
• Locus tag: EST51_RS25470
• Protein ID: WP_001139207.1
• Coordinates: 1822..2073 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EST51_RS25450	1..873	+	873	WP_000729440.1	incFII family plasmid replication initiator RepA	-
EST51_RS27085	1568..1735	+	168	WP_000841010.1	hypothetical protein	-
EST51_RS25470	1822..2073	+	252	WP_001139207.1	plasmid stabilization protein	Antitoxin
EST51_RS25475	2070..2357	+	288	WP_000222775.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EST51_RS27090	2367..2912	+	546	WP_000926984.1	hypothetical protein	-
EST51_RS25500	3564..4166	-	603	WP_000517694.1	hypothetical protein	-
EST51_RS25510	4520..5866	+	1347	WP_087891097.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..97726	97726

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11142.17 Da
• Isoelectric Point: 10.6261

>T125243 WP_000222775.1 NZ_CP040264:2070-2357 [Escherichia coli]
MTYTVKFRDDALREWLKLDKSVQQQFAKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSHVYNLASERMR

Nucleotide

• Length: 288 bp

>T125243 NZ_CP040264:2070-2357 [Escherichia coli]
ATGACTTATACGGTTAAATTCAGGGATGATGCCCTCAGGGAATGGCTGAAACTGGATAAATCCGTTCAACAACAGTTCGC
AAAAAAACTAAAAAAATGCAGCGAAAATCCTCATATACCTTCAGCAAAACTACGAGGATTAAAAGATTGCTACAAAATAA
AACTTCGCGCATCAGGTTTTCGTCTGGTCTATCAGGTGATTGATGACATGTTAATTATTGCGGTTGTCGCAGTAGGTAAA
CGTGAACGTAGTCATGTTTACAATCTCGCCAGCGAGAGAATGAGATAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9216.45 Da
• Isoelectric Point: 4.0927

>AT125243 WP_001139207.1 NZ_CP040264:1822-2073 [Escherichia coli]
MPSIILSDTSASVSELKKNPMATVSAGEGYPVAILNRNQPAFYCVPAELYERMLDAMDDQELVKLVTERSNQPLHDVDLD
SYL

Nucleotide

• Length: 252 bp

>AT125243 NZ_CP040264:1822-2073 [Escherichia coli]
ATGCCCAGTATCATTTTGTCTGACACCAGTGCAAGTGTCAGTGAACTGAAAAAAAATCCAATGGCGACAGTCAGTGCCGG
AGAGGGGTATCCTGTTGCCATTCTTAACCGGAATCAGCCTGCATTCTACTGTGTTCCTGCTGAATTGTATGAAAGGATGC
TTGATGCTATGGATGACCAGGAGCTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTACATGATGTGGACCTGGAT
AGTTATTTATGA

Structures

Toxin

• AlphaFold DB: T2HS21

Antitoxin

• AlphaFold DB: A0A754EQ65