Detailed information of TA system TA125238

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4457419..4458239
• Replicon: chromosome
• Accession: NZ_CP040263
• Organism: Escherichia coli strain 631

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: A0A0V9MKL8
• Locus tag: EST51_RS21950
• Protein ID: WP_001550554.1
• Coordinates: 4457419..4457676 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: -
• Locus tag: EST51_RS21955
• Protein ID: WP_001519303.1
• Coordinates: 4457688..4458239 (+)
• Length: 184 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EST51_RS21930	4452705..4453811	+	1107	WP_001519308.1	N-acetylneuraminate epimerase	-
EST51_RS21935	4453876..4454856	+	981	WP_001519307.1	transposase	-
EST51_RS21940	4455439..4456680	-	1242	Protein_4211	DNA helicase	-
EST51_RS21945	4456743..4457042	+	300	WP_001519304.1	GNAT family N-acetyltransferase	-
EST51_RS21950	4457419..4457676	+	258	WP_001550554.1	hypothetical protein	Toxin
EST51_RS21955	4457688..4458239	+	552	WP_001519303.1	N-acetyltransferase	Antitoxin
EST51_RS27035	4458291..4458452	+	162	WP_001519302.1	hypothetical protein	-
EST51_RS21960	4458489..4459037	+	549	WP_001519301.1	hypothetical protein	-
EST51_RS21965	4459164..4459424	+	261	WP_000084876.1	hypothetical protein	-
EST51_RS27040	4459462..4459578	+	117	Protein_4218	VOC family protein	-
EST51_RS21970	4459823..4460944	+	1122	WP_000010833.1	M42 family metallopeptidase	-
EST51_RS21975	4460941..4461219	+	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
EST51_RS21980	4461231..4462544	+	1314	WP_001519300.1	galactitol-specific PTS transporter subunit IIC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimB	4449903..4458452	8549

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9744.33 Da
• Isoelectric Point: 11.0090

>T125238 WP_001550554.1 NZ_CP040263:4457419-4457676 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKPVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T125238 NZ_CP040263:4457419-4457676 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGCCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 184 a.a.
• Molecular weight: 20463.61 Da
• Isoelectric Point: 6.8758

>AT125238 WP_001519303.1 NZ_CP040263:4457688-4458239 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCAQALMKPEHWRE

Nucleotide

• Length: 552 bp

>AT125238 NZ_CP040263:4457688-4458239 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCGACCTTCAAAGGAGAAATGGATTCACCGCTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCGCAGGCCCTGATGAAACCGGAGCACTGGCGTGAATAA

Structures

Toxin

• AlphaFold DB: A0A0V9MKL8