Detailed information of TA system TA125235

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 3990127..3991816
• Replicon: chromosome
• Accession: NZ_CP040263
• Organism: Escherichia coli strain 631

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: V0Z9M0
• Locus tag: EST51_RS19585
• Protein ID: WP_000365809.1
• Coordinates: 3990127..3991047 (-)
• Length: 307 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: EST51_RS19590
• Protein ID: WP_001550661.1
• Coordinates: 3991034..3991816 (-)
• Length: 261 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EST51_RS19565	3986217..3987344	-	1128	WP_001550663.1	MFS transporter	-
EST51_RS19570	3987486..3988544	-	1059	WP_122987197.1	alpha/beta hydrolase	-
EST51_RS19575	3988809..3989693	+	885	WP_001119037.1	LysR family transcriptional regulator	-
EST51_RS19585	3990127..3991047	-	921	WP_000365809.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
EST51_RS19590	3991034..3991816	-	783	WP_001550661.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
EST51_RS19595	3992132..3995341	+	3210	WP_001521910.1	RNA-directed DNA polymerase	-
EST51_RS19600	3995502..3995642	+	141	WP_001308408.1	ash family protein	-
EST51_RS19605	3995688..3996158	+	471	WP_000201185.1	hypothetical protein	-
EST51_RS19610	3996155..3996257	+	103	Protein_3764	helix-turn-helix domain-containing protein	-
EST51_RS19615	3996349..3996507	+	159	Protein_3765	integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-261)

Antitoxin

• Transcriptional regulator, AbiEi 3 antitoxin, Type IV TA system (95-247)

Sequences

Toxin

Protein

• Length: 307 a.a.
• Molecular weight: 34571.01 Da
• Isoelectric Point: 7.2649

>T125235 WP_000365809.1 NZ_CP040263:c3991047-3990127 [Escherichia coli]
MDLRDSYARQVKLLMAALPYVAKESCFALKGGTAINLFVQDFPRLSVDIDLAYKSFADRDTDLAAINDALKRITASLNGR
PGITAIRQENKADEKRIIVNTADAKIKIEVSPVWRGLLLPPAEMPVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQH
PRDLFDVLNMLEKPGLMREIFDGFLCYLAGHPRPIAELLAPNWDTERIATLYAQEFSGMTQQETSLESLLSVTTLLPQAL
KSHLTDLDREFLLSFKQNSPDWSRYRYPEIQHLPAIRWKQRNLAVLKDKNPAKYVAAVNKLERVLA

Nucleotide

• Length: 921 bp

>T125235 NZ_CP040263:c3991047-3990127 [Escherichia coli]
ATGGATCTGCGTGATAGCTATGCCCGGCAGGTCAAACTGTTGATGGCCGCTCTGCCGTATGTGGCGAAGGAGTCCTGTTT
CGCGTTGAAAGGCGGCACCGCAATCAATTTGTTTGTGCAGGATTTTCCCCGTCTGTCGGTGGATATTGATCTGGCGTACA
AGAGCTTTGCGGATCGCGATACTGATTTGGCGGCTATCAACGACGCTTTGAAGAGGATTACTGCATCGCTGAACGGCAGA
CCGGGGATCACCGCGATTCGGCAGGAAAATAAAGCGGATGAAAAGCGCATCATTGTGAATACCGCTGATGCAAAGATTAA
GATCGAGGTTAGCCCGGTCTGGCGCGGGCTGTTGTTGCCTCCGGCAGAAATGCCTGTGTGCGAACGTGTGGAAATGGAGT
ATGGCTTCACCACCATGAGTGTCGTCTCGCTGGCGGATCTTTATGGCGGTAAAATCTGCGCTGCGCTTGATCGTCAGCAC
CCGCGCGATCTGTTTGATGTGCTGAATATGCTGGAGAAACCGGGACTGATGCGGGAGATCTTTGACGGTTTTCTTTGTTA
TCTGGCAGGCCATCCCCGCCCCATCGCCGAGTTGCTGGCTCCAAACTGGGATACAGAGCGGATCGCCACGTTGTACGCGC
AGGAATTTTCAGGGATGACGCAACAGGAAACGTCGCTGGAATCGCTTCTGTCAGTAACGACTTTGCTGCCGCAGGCGTTG
AAGTCACATCTTACGGATCTGGATCGCGAGTTCTTGCTCAGTTTTAAGCAAAACAGCCCTGACTGGTCGCGGTATCGCTA
TCCTGAAATTCAGCATCTTCCGGCTATTCGCTGGAAGCAACGCAATCTGGCGGTGCTTAAAGATAAAAATCCGGCGAAGT
ATGTGGCGGCGGTGAATAAGCTAGAGAGGGTTCTGGCGTAA

Antitoxin

Protein

• Length: 261 a.a.
• Molecular weight: 29191.43 Da
• Isoelectric Point: 8.9059

>AT125235 WP_001550661.1 NZ_CP040263:c3991816-3991034 [Escherichia coli]
MPNYLNWLMQHTLPGQVLLQSWLTRNGIDRKLSYLYVKNGWLRRIAHGVYCRTGREPDWVDAVYCLQSQWEKPVRVAGLT
SLALQGHAHYTEQGKPQVWLALPAYVKLPGWFSAFEQSATFVTLYTSGLTADVTPFTTELKIGDRQLAGSVPELAAYEIA
CGVPSLISFEHADALFQGLNLLSPRKLQALLRASHSVKTNRVMLYLARRNGHPYFQYIDQSGIETGTGKRQIIPGGWLEP
DYQITVPRAFKPEGVEDGSA

Nucleotide

• Length: 783 bp

>AT125235 NZ_CP040263:c3991816-3991034 [Escherichia coli]
TTGCCAAACTATCTAAACTGGCTGATGCAGCACACGCTACCCGGCCAGGTGCTGCTCCAGTCATGGCTTACCCGCAACGG
CATAGATCGTAAGCTCTCTTATCTCTATGTGAAGAATGGCTGGCTCAGGCGGATCGCTCACGGCGTATACTGCCGGACGG
GTCGTGAACCGGATTGGGTGGATGCGGTTTACTGCCTGCAATCGCAGTGGGAAAAGCCTGTTCGGGTGGCGGGGCTAACC
AGCCTTGCGCTTCAGGGTCATGCGCACTATACCGAACAGGGTAAGCCGCAGGTCTGGCTCGCGCTTCCGGCTTACGTCAA
ACTGCCGGGATGGTTTTCTGCCTTTGAACAATCCGCAACGTTTGTCACGCTGTATACGTCAGGCCTTACCGCTGATGTAA
CGCCATTTACTACGGAACTGAAAATTGGCGACCGCCAGTTAGCAGGCAGTGTTCCGGAACTGGCCGCGTATGAGATCGCC
TGCGGCGTTCCTTCGTTAATCTCGTTCGAACATGCCGATGCTCTGTTTCAGGGGTTGAATCTTCTCAGCCCACGCAAATT
GCAGGCACTGCTTCGCGCCAGCCATTCGGTTAAAACCAACCGGGTGATGCTTTACCTTGCCCGGCGTAATGGACACCCTT
ATTTTCAGTATATTGATCAAAGCGGTATAGAAACAGGTACGGGAAAGCGGCAAATCATACCCGGCGGCTGGCTGGAGCCG
GATTATCAAATTACTGTTCCACGCGCATTTAAACCGGAAGGAGTCGAAGATGGATCTGCGTGA

Structures

Toxin

• AlphaFold DB: V0Z9M0