Detailed information of TA system TA125217

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 829101..829936
• Replicon: chromosome
• Accession: NZ_CP040263
• Organism: Escherichia coli strain 631

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0A1A9A5
• Locus tag: EST51_RS03995
• Protein ID: WP_001564063.1
• Coordinates: 829101..829478 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0K5N986
• Locus tag: EST51_RS04000
• Protein ID: WP_021553056.1
• Coordinates: 829568..829936 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EST51_RS03965	824229..825377	-	1149	WP_000905920.1	capsule polysaccharide export inner-membrane protein KpsE	-
EST51_RS03970	825449..826432	-	984	WP_001296394.1	KpsF/GutQ family sugar-phosphate isomerase	-
EST51_RS03975	827242..827412	-	171	Protein_764	IS110 family transposase	-
EST51_RS03980	827755..828597	-	843	Protein_765	DUF4942 domain-containing protein	-
EST51_RS26895	828682..829104	-	423	WP_001564064.1	DUF957 domain-containing protein	-
EST51_RS03995	829101..829478	-	378	WP_001564063.1	TA system toxin CbtA family protein	Toxin
EST51_RS04000	829568..829936	-	369	WP_021553056.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
EST51_RS04005	830099..830320	-	222	WP_000692345.1	DUF987 domain-containing protein	-
EST51_RS04010	830385..830861	-	477	WP_021553055.1	RadC family protein	-
EST51_RS04015	830877..831356	-	480	WP_001564060.1	antirestriction protein	-
EST51_RS04020	831622..832440	-	819	WP_001564059.1	DUF945 domain-containing protein	-
EST51_RS04025	832530..832763	-	234	WP_001278293.1	DUF905 family protein	-
EST51_RS04030	832769..833446	-	678	WP_001564058.1	hypothetical protein	-
EST51_RS04035	833597..834277	-	681	WP_001278649.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	papX	827628..901209	73581

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14094.09 Da
• Isoelectric Point: 7.8045

>T125217 WP_001564063.1 NZ_CP040263:c829478-829101 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYVLVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRSNYRTVNNITRGKHPEAKQ

Nucleotide

• Length: 378 bp

>T125217 NZ_CP040263:c829478-829101 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGTACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGTCTGATGACCCGCAG
CAACTACAGAACGGTAAATAACATTACCCGGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13615.39 Da
• Isoelectric Point: 7.0264

>AT125217 WP_021553056.1 NZ_CP040263:c829936-829568 [Escherichia coli]
VSDTLSGTTHPDDNHDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGNRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT125217 NZ_CP040263:c829936-829568 [Escherichia coli]
GTGTCAGACACACTCTCCGGGACAACGCATCCCGATGACAATCACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTAACAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A0A1A9A5

Antitoxin

• AlphaFold DB: A0A0K5N986