Detailed information of TA system TA125174

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1476942..1477719
• Replicon: chromosome
• Accession: NZ_CP040243
• Organism: Glaesserella parasuis strain HPS-1

Toxin (Protein)

• Gene name: TacT3
• Uniprot ID: -
• Locus tag: FEF05_RS07385
• Protein ID: WP_149350996.1
• Coordinates: 1477219..1477719 (+)
• Length: 167 a.a.

Antitoxin (Protein)

• Gene name: TacA3
• Uniprot ID: U4SPD7
• Locus tag: FEF05_RS07380
• Protein ID: WP_005710653.1
• Coordinates: 1476942..1477229 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FEF05_RS07360	1473308..1473808	-	501	WP_035519842.1	CvpA family protein	-
FEF05_RS07365	1473914..1475491	-	1578	WP_005710646.1	FAD-binding protein	-
FEF05_RS07370	1475666..1476403	-	738	WP_149350994.1	peptidoglycan editing factor PgeF	-
FEF05_RS07375	1476407..1476796	-	390	WP_149350995.1	RidA family protein	-
FEF05_RS07380	1476942..1477229	+	288	WP_005710653.1	DUF1778 domain-containing protein	Antitoxin
FEF05_RS07385	1477219..1477719	+	501	WP_149350996.1	GNAT family N-acetyltransferase	Toxin
FEF05_RS07390	1477747..1478748	+	1002	WP_010786647.1	nucleoid-associated protein YejK	-
FEF05_RS07395	1478809..1479144	+	336	WP_005710658.1	outer membrane protein assembly factor BamE	-
FEF05_RS07400	1479299..1480414	+	1116	WP_044009508.1	anhydro-N-acetylmuramic acid kinase	-
FEF05_RS07405	1480426..1481340	+	915	WP_149350997.1	N-acetylmuramic acid 6-phosphate etherase	-
FEF05_RS07410	1481382..1481696	+	315	WP_005710664.1	YqcC family protein	-
FEF05_RS07415	1481693..1482421	+	729	WP_021115014.1	tRNA pseudouridine(65) synthase TruC	-
FEF05_RS07420	1482439..1482594	+	156	WP_005710668.1	YoaH family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (4-87)

Sequences

Toxin

Protein

• Length: 167 a.a.
• Molecular weight: 19056.21 Da
• Isoelectric Point: 9.0168

>T125174 WP_149350996.1 NZ_CP040243:1477219-1477719 [Glaesserella parasuis]
MKSKFIEEPLTKHHQREAFDCGNLEMNRFFKQYARQSHEKGTSKTYISRHLDSQEIIGFYTITLSALDQQHLPELCKKRF
GYHPIPLFTLARLAVDIRYQKQGIGGMLLVKALKRCAIVAEQVGGIGLLIEAKDEDISRWYQSYGAIPLENMPLTLILLF
DTIKGL

Nucleotide

• Length: 501 bp

>T125174 NZ_CP040243:1477219-1477719 [Glaesserella parasuis]
ATGAAGAGTAAGTTTATTGAAGAGCCTTTAACGAAGCATCATCAACGAGAAGCCTTTGATTGCGGAAATCTAGAGATGAA
CCGATTTTTTAAACAATACGCTCGGCAATCTCACGAAAAAGGGACATCAAAAACTTACATTAGCCGACACTTAGACAGTC
AAGAGATTATCGGATTTTATACAATCACCTTAAGTGCATTAGACCAGCAACACTTACCCGAACTATGCAAAAAACGTTTT
GGCTACCACCCTATTCCGCTCTTTACATTAGCAAGACTTGCAGTGGATATTCGCTATCAAAAACAAGGCATTGGCGGAAT
GCTGTTGGTTAAAGCTCTCAAACGCTGTGCCATCGTTGCCGAACAAGTCGGGGGAATTGGGCTTCTCATTGAAGCAAAAG
ATGAAGACATTTCTCGTTGGTATCAATCTTATGGTGCTATTCCATTGGAAAATATGCCATTAACCTTAATTCTACTCTTT
GATACAATCAAAGGACTTTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10917.47 Da
• Isoelectric Point: 6.4650

>AT125174 WP_005710653.1 NZ_CP040243:1476942-1477229 [Glaesserella parasuis]
MPQIANALNDRMSIRISTEDKQTLIRAVALTHTNITEFVLQRILPSAREIIQQYDNRQFSPQDLSLIMELLDNPPKANTK
LQQAAKLAKESYHEE

Nucleotide

• Length: 288 bp

>AT125174 NZ_CP040243:1476942-1477229 [Glaesserella parasuis]
ATGCCACAAATTGCAAATGCCCTTAACGATAGAATGTCTATTCGCATTAGTACGGAAGACAAGCAGACTTTAATTAGAGC
CGTTGCACTTACCCATACGAATATTACGGAATTTGTATTACAACGTATTTTGCCTAGTGCCAGAGAAATTATTCAGCAGT
ATGATAATCGGCAATTTTCACCGCAAGATCTCTCGTTGATTATGGAATTGCTTGATAATCCGCCGAAAGCTAATACAAAA
CTACAACAAGCGGCGAAATTAGCAAAGGAAAGCTATCATGAAGAGTAA

Structures

Antitoxin

• AlphaFold DB: U4SPD7