Detailed information of TA system TA125166

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-epsilon
• Location: 155390..156527
• Replicon: plasmid unnamed1
• Accession: NZ_CP040237
• Organism: Enterococcus faecium strain VB3025

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: -
• Locus tag: FBF65_RS15380
• Protein ID: WP_002401483.1
• Coordinates: 155390..156253 (-)
• Length: 288 a.a.

Antitoxin (Protein)

• Gene name: epsilon
• Uniprot ID: R2XCR7
• Locus tag: FBF65_RS15385
• Protein ID: WP_000301765.1
• Coordinates: 156255..156527 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FBF65_RS15350 (FBF65_15350)	151284..152774	-	1491	WP_002380753.1	primase C-terminal domain-containing protein	-
FBF65_RS15355 (FBF65_15355)	153168..153407	-	240	WP_002380754.1	helix-turn-helix domain-containing protein	-
FBF65_RS15365 (FBF65_15365)	153769..154506	-	738	WP_001038792.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B)	-
FBF65_RS15370 (FBF65_15370)	154631..154714	-	84	WP_001814874.1	23S rRNA methyltransferase attenuation leader peptide	-
FBF65_RS15380 (FBF65_15380)	155390..156253	-	864	WP_002401483.1	zeta toxin family protein	Toxin
FBF65_RS15385 (FBF65_15385)	156255..156527	-	273	WP_000301765.1	antitoxin	Antitoxin
FBF65_RS15390 (FBF65_15390)	156544..156759	-	216	WP_002295735.1	peptide-binding protein	-
FBF65_RS15395 (FBF65_15395)	156830..157726	-	897	WP_118215883.1	ParA family protein	-
FBF65_RS15400 (FBF65_15400)	157829..158089	-	261	Protein_163	topoisomerase DNA-binding C4 zinc finger domain-containing protein	-
FBF65_RS15410 (FBF65_15410)	158259..158996	-	738	WP_001038792.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B)	-
FBF65_RS15415 (FBF65_15415)	159121..159216	-	96	WP_001809736.1	23S rRNA methyltransferase attenuation leader peptide	-
FBF65_RS15420 (FBF65_15420)	159265..159504	-	240	WP_000635249.1	peptide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(6')-aph(2'') / erm(B)	-	1..210997	210997
	-	inside	IScluster/Tn	aac(6')-aph(2'') / erm(B)	-	146814..158996	12182

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (27-213)

Antitoxin

• Bacterial epsilon antitoxin (2-90)

Sequences

Toxin

Protein

• Length: 288 a.a.
• Molecular weight: 32486.06 Da
• Isoelectric Point: 6.9965

>T125166 WP_002401483.1 NZ_CP040237:c156253-155390 [Enterococcus faecium]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAILEETQGNVIVIDNDTFKQQHPNFDELA
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSRLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMLQNQHQETPEFKAIQQKMESLQPPTPPIPKTPKLPGL

Nucleotide

• Length: 864 bp

>T125166 NZ_CP040237:c156253-155390 [Enterococcus faecium]
ATGGCAAATATAGTCAATTTTACTGACAAACAATTTGAGAATCGCTTAAATGATAATTTAGAAGAATTGATTCAAGGAAA
AAAAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGGAAAACCAGTTTGCGATCAGCAATTC
TTGAAGAAACACAAGGGAATGTTATTGTCATTGATAATGATACCTTTAAACAACAGCACCCTAATTTTGATGAACTAGCG
AAACTTTATGAGAAAGACGTAGTAAAACACGTCACCCCTTATTCTAATCGCATGACAGAAGCGATCATAAGCCGTTTGAG
CGATCAAGGCTATAATTTAGTGATTGAAGGTACAGGACGAACAACAGACGTTCCTATTCAAACCGCAACAATGCTTCAAG
CCAAAGGTTATGAAACAAAAATGTATGTTATGGCAGTACCTAAAATCAACTCATATTTAGGAACAATTGAACGATATGAA
ACTATGTATGCAGATGATCCAATGACAGCCAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTTTACAATAGAGAAGGAGTGAAACTCTATTCAA
GATTAGAAACGCCTTCTATTAGTCCGAAAGAGACCTTAGAAAGAGAATTAAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTGGAGAGAATAGAGCAAAAAATGCTTCAAAATCAACACCAAGAAACCCCTGAATTTAAAGCAATTCAACAAAA
AATGGAAAGCTTGCAGCCACCTACACCACCAATACCCAAAACACCTAAACTTCCAGGACTTTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10721.25 Da
• Isoelectric Point: 6.4676

>AT125166 WP_000301765.1 NZ_CP040237:c156527-156255 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Nucleotide

• Length: 273 bp

>AT125166 NZ_CP040237:c156527-156255 [Enterococcus faecium]
ATGGCAGTTACATATGAAAAAACATTTGAAATTGAAATCATTAATGAGTTATCGGCAAGTGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAATTAAATAAAAATGACTCTCAATTATTGGAAGTCAATTTATTAAACCAATTAAAGCTTGCAA
AACGTGTAAATCTTTTTGATTATTCTTTAGAAGAATTACAAGCCGTTCATGAGTATTGGCGGTCAATGAATCGTTACTCA
AAACAAGTTTTGAATAAAGAGAAAGTGGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A829F0A3