Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 651221..651792 Replicon chromosome
Accession NZ_CP040236
Organism Enterococcus faecium strain VB3025

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag FBF65_RS03330 Protein ID WP_002286801.1
Coordinates 651451..651792 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag FBF65_RS03325 Protein ID WP_002323011.1
Coordinates 651221..651451 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FBF65_RS03280 (FBF65_03280) 646356..647687 + 1332 WP_002286818.1 FAD-containing oxidoreductase -
FBF65_RS03285 (FBF65_03285) 647709..648335 + 627 WP_002286816.1 cysteine hydrolase -
FBF65_RS03290 (FBF65_03290) 648518..649099 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
FBF65_RS03310 (FBF65_03310) 649831..650391 + 561 WP_138013253.1 SOS response-associated peptidase family protein -
FBF65_RS03315 (FBF65_03315) 650596..650934 - 339 WP_002286804.1 hypothetical protein -
FBF65_RS03325 (FBF65_03325) 651221..651451 + 231 WP_002323011.1 hypothetical protein Antitoxin
FBF65_RS03330 (FBF65_03330) 651451..651792 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
FBF65_RS03335 (FBF65_03335) 652642..652827 + 186 WP_002304207.1 hypothetical protein -
FBF65_RS03340 (FBF65_03340) 653332..653526 + 195 WP_002297028.1 hypothetical protein -
FBF65_RS03345 (FBF65_03345) 653717..653966 + 250 Protein_609 transposase -
FBF65_RS03355 (FBF65_03355) 654210..655040 - 831 WP_002286789.1 manganese catalase family protein -
FBF65_RS03360 (FBF65_03360) 655248..656582 + 1335 WP_002286788.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T125165 WP_002286801.1 NZ_CP040236:651451-651792 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T125165 NZ_CP040236:651451-651792 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT125165 WP_002323011.1 NZ_CP040236:651221-651451 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT125165 NZ_CP040236:651221-651451 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References