Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relE-yefM/Txe-RelB
Location 331112..331641 Replicon chromosome
Accession NZ_CP040236
Organism Enterococcus faecium strain VB3025

Toxin (Protein)


Gene name relE Uniprot ID Q3XX67
Locus tag FBF65_RS01725 Protein ID WP_002296560.1
Coordinates 331375..331641 (+) Length 89 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A829F3T0
Locus tag FBF65_RS01720 Protein ID WP_002296559.1
Coordinates 331112..331375 (+) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FBF65_RS01705 (FBF65_01705) 328486..330309 + 1824 WP_002294202.1 APC family permease -
FBF65_RS01710 (FBF65_01710) 330451..330636 - 186 WP_002296558.1 transcriptional regulator -
FBF65_RS01720 (FBF65_01720) 331112..331375 + 264 WP_002296559.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
FBF65_RS01725 (FBF65_01725) 331375..331641 + 267 WP_002296560.1 Txe/YoeB family addiction module toxin Toxin
FBF65_RS01730 (FBF65_01730) 331842..332330 + 489 WP_002296561.1 mannosyl-glycoprotein endo-beta-N-acetylglucosamidase -
FBF65_RS01735 (FBF65_01735) 332871..333524 - 654 WP_002323018.1 ABC transporter permease -
FBF65_RS01740 (FBF65_01740) 333539..334456 - 918 WP_002296564.1 osmoprotectant ABC transporter substrate-binding protein -
FBF65_RS01745 (FBF65_01745) 334453..335094 - 642 WP_085811560.1 ABC transporter permease -
FBF65_RS01750 (FBF65_01750) 335098..336273 - 1176 WP_002294192.1 betaine/proline/choline family ABC transporter ATP-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(9-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-69)


Sequences

Toxin        


Download         Length: 89 a.a.        Molecular weight: 10660.13 Da        Isoelectric Point: 9.9931

>T125164 WP_002296560.1 NZ_CP040236:331375-331641 [Enterococcus faecium]
MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQSFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEI
YSAWTHYE

Download         Length: 267 bp

>T125164 NZ_CP040236:331375-331641 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAAACGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTAGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10046.05 Da        Isoelectric Point: 4.1207

>AT125164 WP_002296559.1 NZ_CP040236:331112-331375 [Enterococcus faecium]
MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIGLEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNID
DIDWEAL

Download         Length: 264 bp

>AT125164 NZ_CP040236:331112-331375 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACTGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTGGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTAGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FCW8

Antitoxin

Source ID Structure
AlphaFold DB A0A829F3T0

References