Detailed information of TA system TA125161

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4390849..4391465
• Replicon: chromosome
• Accession: NZ_CP040234
• Organism: Citrobacter sp. TBCP-5362

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A8AL49
• Locus tag: FD428_RS20845
• Protein ID: WP_012133919.1
• Coordinates: 4390849..4391223 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: FD428_RS20850
• Protein ID: WP_162790489.1
• Coordinates: 4391220..4391465 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FD428_RS20830	4388352..4389254	+	903	WP_071259455.1	formate dehydrogenase O subunit beta	-
FD428_RS20835	4389251..4389886	+	636	WP_049011887.1	formate dehydrogenase cytochrome b556 subunit	-
FD428_RS20840	4389883..4390812	+	930	WP_047459875.1	formate dehydrogenase accessory protein FdhE	-
FD428_RS20845	4390849..4391223	-	375	WP_012133919.1	type II toxin-antitoxin system VapC family toxin	Toxin
FD428_RS20850	4391220..4391465	-	246	WP_162790489.1	ribbon-helix-helix domain-containing protein	Antitoxin
FD428_RS20855	4391701..4392642	-	942	WP_024130670.1	fatty acid biosynthesis protein FabY	-
FD428_RS20860	4392687..4393124	-	438	WP_047459887.1	D-tyrosyl-tRNA(Tyr) deacylase	-
FD428_RS20865	4393121..4393993	-	873	WP_012133924.1	virulence factor BrkB family protein	-
FD428_RS20870	4393987..4394586	-	600	WP_047459890.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-112)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-47)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13741.00 Da
• Isoelectric Point: 9.8061

>T125161 WP_012133919.1 NZ_CP040234:c4391223-4390849 [Citrobacter sp. TBCP-5362]
MIKGPVLFDTNILIDLFSGRGEAKKALEAYSPQNAISLITWMEVMVGAKKYHQESRTRIALSAFNVIGVSQDIAERSVSL
RQEYGMKLPDAVILATAHIHRFALVTRNTKDFAGIPGVITPYHL

Nucleotide

• Length: 375 bp

>T125161 NZ_CP040234:c4391223-4390849 [Citrobacter sp. TBCP-5362]
ATGATTAAAGGTCCGGTGTTGTTTGACACCAATATTCTCATTGATTTATTTAGCGGGCGTGGTGAAGCGAAGAAGGCGCT
CGAAGCTTACTCACCCCAGAATGCCATTAGTCTCATTACCTGGATGGAAGTGATGGTAGGGGCAAAAAAGTACCATCAGG
AATCCCGCACACGTATAGCATTGAGCGCTTTTAACGTTATTGGCGTTTCACAGGATATTGCCGAAAGAAGTGTCAGTCTC
AGGCAGGAATACGGGATGAAGTTGCCGGATGCGGTTATTCTGGCTACAGCGCACATTCATCGTTTTGCGCTCGTGACGCG
CAATACAAAGGACTTTGCGGGGATTCCGGGAGTCATTACGCCTTACCATTTATAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9524.75 Da
• Isoelectric Point: 4.1961

>AT125161 WP_162790489.1 NZ_CP040234:c4391465-4391220 [Citrobacter sp. TBCP-5362]
MMAGMEMGRILLDLSDEVIKRLDDLKQQRNLPRAELLREAVEQYLERQGQAETTISDALGLWQGCEEDGVEYERKLREEW
Q

Nucleotide

• Length: 246 bp

>AT125161 NZ_CP040234:c4391465-4391220 [Citrobacter sp. TBCP-5362]
ATGATGGCTGGAATGGAAATGGGCAGAATATTACTTGATTTATCAGATGAGGTTATCAAGCGGCTTGATGACCTCAAACA
ACAGCGTAATCTTCCGCGAGCAGAGCTATTACGCGAAGCAGTTGAACAATACCTGGAAAGGCAGGGACAAGCCGAAACCA
CTATTTCCGATGCGCTGGGGCTCTGGCAAGGTTGCGAAGAAGATGGCGTTGAATACGAAAGGAAGCTCCGCGAGGAGTGG
CAATGA

Structures

Toxin

• AlphaFold DB: A8AL49