Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3339809..3340429 Replicon chromosome
Accession NZ_CP040234
Organism Citrobacter sp. TBCP-5362

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag FD428_RS15930 Protein ID WP_002892050.1
Coordinates 3340211..3340429 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A8AJY4
Locus tag FD428_RS15925 Protein ID WP_012133514.1
Coordinates 3339809..3340183 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FD428_RS15915 3334945..3336138 + 1194 WP_012133512.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
FD428_RS15920 3336161..3339307 + 3147 WP_012133513.1 efflux RND transporter permease AcrB -
FD428_RS15925 3339809..3340183 + 375 WP_012133514.1 Hha toxicity modulator TomB Antitoxin
FD428_RS15930 3340211..3340429 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
FD428_RS15935 3340613..3341164 + 552 WP_049009805.1 maltose O-acetyltransferase -
FD428_RS15940 3341278..3341748 + 471 WP_114847774.1 hypothetical protein -
FD428_RS15945 3341826..3341966 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
FD428_RS15950 3341968..3342228 - 261 WP_012133519.1 type B 50S ribosomal protein L31 -
FD428_RS15955 3342454..3344004 + 1551 WP_114847716.1 EAL domain-containing protein -
FD428_RS15960 3344044..3344397 - 354 WP_012133521.1 DUF1428 family protein -
FD428_RS15965 3344477..3345091 - 615 WP_101743908.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3339809..3347853 8044


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T125160 WP_002892050.1 NZ_CP040234:3340211-3340429 [Citrobacter sp. TBCP-5362]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T125160 NZ_CP040234:3340211-3340429 [Citrobacter sp. TBCP-5362]
ATGTCCGATAAACCATTAACGAAAACAGATTATTTGATGCGTTTACGACGCTGCCAGACAATAGACACGCTGGAACGTGT
AATAGAGAAAAATAAATATGAATTGTCTGACAATGAATTGGCTGTATTTTACTCAGCAGCAGATCACCGTCTTGCAGAAT
TGACGATGAATAAGCTCTACGACAAGATTCCCACCTCCGTGTGGAAATTCATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14437.27 Da        Isoelectric Point: 5.1444

>AT125160 WP_012133514.1 NZ_CP040234:3339809-3340183 [Citrobacter sp. TBCP-5362]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT125160 NZ_CP040234:3339809-3340183 [Citrobacter sp. TBCP-5362]
ATGGATGAGTACTCACCCAAAAGACATGATATCGCACAGCTTAAATTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCACGGTTGGGTTAATGACCCTACTTCGGCAATCAATCTTCAGCTCAATGAACTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTATAATGAGGACAATAAGCTGATTGCACAGATAGATGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTACGGCATTAATGCGCAGGATCTGCAAAAATGGCGAAAATCAGGAAACCGCCT
GTTTCGCTGCTTCGTCAATGCCACCAGGGCGAATCCTGTTAGCTTGTCATGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A8AJY4

References