Detailed information of TA system TA125150

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 1091355..1092021
• Replicon: chromosome
• Accession: NZ_CP040234
• Organism: Citrobacter sp. TBCP-5362

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: FD428_RS05285
• Protein ID: WP_114848638.1
• Coordinates: 1091355..1091684 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: FD428_RS05290
• Protein ID: WP_114848637.1
• Coordinates: 1091704..1092021 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FD428_RS05265	1086499..1086678	-	180	WP_114848641.1	DinI family protein	-
FD428_RS05270	1087213..1087775	+	563	Protein_1014	phage polarity suppression protein	-
FD428_RS05275	1088018..1089748	-	1731	WP_114848640.1	AIPR family protein	-
FD428_RS05280	1089762..1090961	-	1200	WP_114848639.1	tyrosine-type recombinase/integrase	-
FD428_RS05285	1091355..1091684	-	330	WP_114848638.1	TA system toxin CbtA family protein	Toxin
FD428_RS05290	1091704..1092021	-	318	WP_114848637.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
FD428_RS05295	1092040..1092261	-	222	WP_016248358.1	DUF987 domain-containing protein	-
FD428_RS05300	1092270..1092746	-	477	WP_114848636.1	RadC family protein	-
FD428_RS05305	1092762..1093220	-	459	WP_114848635.1	antirestriction protein	-
FD428_RS05310	1093306..1093542	-	237	WP_114848634.1	DUF905 domain-containing protein	-
FD428_RS05315	1093620..1094030	-	411	WP_114848633.1	hypothetical protein	-
FD428_RS05320	1094096..1094533	-	438	WP_047028252.1	hypothetical protein	-
FD428_RS05325	1094575..1095111	-	537	WP_114848632.1	DUF4339 domain-containing protein	-
FD428_RS05330	1095108..1095836	-	729	WP_114848631.1	DeoR family transcriptional regulator	-
FD428_RS05335	1096051..1096869	-	819	Protein_1027	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1086263..1118168	31905

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-108)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12225.28 Da
• Isoelectric Point: 10.3407

>T125150 WP_114848638.1 NZ_CP040234:c1091684-1091355 [Citrobacter sp. TBCP-5362]
MKTLPATISRAAKPCLSPVAVWQILLTRLLEQHYGLTLSDTPFSDKTVIKEHINAGVSLSDAVNFLVEKYGLVRIDRKGF
SWQEQTPYISVVDILRARRSTGLLKTNVK

Nucleotide

• Length: 330 bp

>T125150 NZ_CP040234:c1091684-1091355 [Citrobacter sp. TBCP-5362]
ATGAAAACTCTACCTGCTACAATTTCGCGGGCGGCGAAGCCCTGTCTGTCGCCCGTAGCTGTCTGGCAAATACTACTGAC
ACGCCTGCTGGAACAGCACTATGGCCTGACACTGAGCGACACGCCGTTCAGTGATAAAACAGTTATTAAGGAACATATTA
ATGCTGGAGTATCGCTCAGCGATGCGGTGAACTTTCTTGTCGAAAAATACGGGCTTGTCCGTATCGACCGAAAGGGATTT
TCGTGGCAAGAACAAACCCCGTATATTTCCGTAGTGGATATTCTGCGAGCAAGGCGCTCTACCGGCTTGCTAAAAACTAA
TGTGAAATAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11944.57 Da
• Isoelectric Point: 6.8412

>AT125150 WP_114848637.1 NZ_CP040234:c1092021-1091704 [Citrobacter sp. TBCP-5362]
MSNTTWGLQRDITPRLGARLVQEGNRLYYLADRASIIGKFSDAECRKLDETFPHFISQMELMLITGELNPRHAHCVNLYH
NGFTCEADTLGSCGYAYIAVYPTQR

Nucleotide

• Length: 318 bp

>AT125150 NZ_CP040234:c1092021-1091704 [Citrobacter sp. TBCP-5362]
ATGAGCAACACCACATGGGGCCTGCAGCGGGATATCACGCCGCGCCTAGGAGCCCGCCTTGTGCAGGAGGGGAACCGGCT
GTATTACTTGGCTGACCGGGCCAGCATCATCGGTAAGTTCAGTGACGCCGAATGCCGGAAACTGGATGAAACTTTCCCGC
ACTTTATCAGCCAGATGGAATTGATGCTAATCACCGGTGAACTGAATCCCCGACATGCCCACTGCGTCAACCTGTACCAC
AATGGTTTCACCTGCGAAGCCGATACCCTTGGTAGTTGCGGCTATGCATACATCGCTGTTTATCCCACTCAACGCTAA