Detailed information of TA system TA125130

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2193526..2194055
• Replicon: chromosome
• Accession: NZ_CP040232
• Organism: Staphylococcus aureus strain GD1706

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: FD746_RS11095
• Protein ID: WP_000621175.1
• Coordinates: 2193526..2193888 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: FD746_RS11100
• Protein ID: WP_000948331.1
• Coordinates: 2193885..2194055 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FD746_RS11075	2190504..2191274	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
FD746_RS11080	2191249..2191728	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
FD746_RS11085	2191730..2192056	-	327	WP_001052491.1	anti-sigma factor antagonist	-
FD746_RS11090	2192175..2193176	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
FD746_RS11095	2193526..2193888	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FD746_RS11100	2193885..2194055	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
FD746_RS11105	2194140..2195288	-	1149	WP_001281139.1	alanine racemase	-
FD746_RS11110	2195354..2195713	-	360	WP_000581197.1	holo-ACP synthase	-
FD746_RS11115	2195717..2196208	-	492	WP_001286980.1	PH domain-containing protein	-
FD746_RS11120	2196201..2197778	-	1578	WP_149888536.1	PH domain-containing protein	-
FD746_RS11125	2197771..2198250	-	480	WP_001287081.1	hypothetical protein	-
FD746_RS11130	2198459..2199019	-	561	WP_001092419.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T125130 WP_000621175.1 NZ_CP040232:c2193888-2193526 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T125130 NZ_CP040232:c2193888-2193526 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAACAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAGCAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTAAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT125130 WP_000948331.1 NZ_CP040232:c2194055-2193885 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT125130 NZ_CP040232:c2194055-2193885 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1