Detailed information of TA system TA125118

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2707131..2707660
• Replicon: chromosome
• Accession: NZ_CP040230
• Organism: Staphylococcus aureus strain GD1108

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: FD491_RS13230
• Protein ID: WP_000621175.1
• Coordinates: 2707298..2707660 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: FD491_RS13225
• Protein ID: WP_000948331.1
• Coordinates: 2707131..2707301 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FD491_RS13195	2702167..2702727	+	561	WP_001092419.1	K(+)-transporting ATPase subunit C	-
FD491_RS13200	2702936..2703415	+	480	WP_001287081.1	hypothetical protein	-
FD491_RS13205	2703408..2704985	+	1578	WP_001294650.1	PH domain-containing protein	-
FD491_RS13210	2704978..2705469	+	492	WP_001286980.1	PH domain-containing protein	-
FD491_RS13215	2705473..2705832	+	360	WP_000581197.1	holo-ACP synthase	-
FD491_RS13220	2705898..2707046	+	1149	WP_001281139.1	alanine racemase	-
FD491_RS13225	2707131..2707301	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
FD491_RS13230	2707298..2707660	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FD491_RS13235	2708010..2709011	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
FD491_RS13240	2709130..2709456	+	327	WP_001052491.1	anti-sigma factor antagonist	-
FD491_RS13245	2709458..2709937	+	480	WP_001190825.1	anti-sigma B factor RsbW	-
FD491_RS13250	2709912..2710682	+	771	WP_001041107.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T125118 WP_000621175.1 NZ_CP040230:2707298-2707660 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T125118 NZ_CP040230:2707298-2707660 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAACAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAGCAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTAAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT125118 WP_000948331.1 NZ_CP040230:2707131-2707301 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT125118 NZ_CP040230:2707131-2707301 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1