Detailed information of TA system TA125099

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2058702..2059231
• Replicon: chromosome
• Accession: NZ_CP040229
• Organism: Staphylococcus aureus strain GD487

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: FD482_RS10125
• Protein ID: WP_000621175.1
• Coordinates: 2058702..2059064 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: FD482_RS10130
• Protein ID: WP_000948331.1
• Coordinates: 2059061..2059231 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FD482_RS10105	2055680..2056450	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
FD482_RS10110	2056425..2056904	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
FD482_RS10115	2056906..2057232	-	327	WP_001052491.1	anti-sigma factor antagonist	-
FD482_RS10120	2057351..2058352	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
FD482_RS10125	2058702..2059064	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FD482_RS10130	2059061..2059231	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
FD482_RS10135	2059316..2060464	-	1149	WP_001281139.1	alanine racemase	-
FD482_RS10140	2060530..2060889	-	360	WP_000581197.1	holo-ACP synthase	-
FD482_RS10145	2060893..2061384	-	492	WP_001286980.1	PH domain-containing protein	-
FD482_RS10150	2061377..2062954	-	1578	WP_001294650.1	PH domain-containing protein	-
FD482_RS10155	2062947..2063426	-	480	WP_138767006.1	hypothetical protein	-
FD482_RS10160	2063635..2064195	-	561	WP_138767007.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T125099 WP_000621175.1 NZ_CP040229:c2059064-2058702 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T125099 NZ_CP040229:c2059064-2058702 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAACAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAGCAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTAAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT125099 WP_000948331.1 NZ_CP040229:c2059231-2059061 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT125099 NZ_CP040229:c2059231-2059061 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1