Detailed information of TA system TA125092

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/ElaA-DUF1778
• Location: 59945..60696
• Replicon: plasmid pRp_3
• Accession: NZ_CP040186
• Organism: Raoultella planticola strain Rp_CZ180511

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: FDZ05_RS29955
• Protein ID: WP_032694614.1
• Coordinates: 59945..60427 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A071LPN3
• Locus tag: FDZ05_RS29960
• Protein ID: WP_004902250.1
• Coordinates: 60418..60696 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FDZ05_RS29935	55135..56634	+	1500	WP_180889157.1	IS21 family transposase	-
FDZ05_RS29940	56631..57386	+	756	WP_038423655.1	IS21-like element ISEc10 family helper ATPase IstB	-
FDZ05_RS29945	57453..58070	-	618	Protein_64	transposase	-
FDZ05_RS29950	58352..59202	-	851	Protein_65	IS3 family transposase	-
FDZ05_RS29955	59945..60427	-	483	WP_032694614.1	GNAT family N-acetyltransferase	Toxin
FDZ05_RS29960	60418..60696	-	279	WP_004902250.1	DUF1778 domain-containing protein	Antitoxin
FDZ05_RS29965	60833..61027	-	195	Protein_68	hypothetical protein	-
FDZ05_RS29970	61111..61452	-	342	WP_032694615.1	hypothetical protein	-
FDZ05_RS29975	61771..62776	-	1006	Protein_70	IS110-like element IS4321 family transposase	-
FDZ05_RS29980	63084..63437	+	354	WP_025376942.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
FDZ05_RS29985	63485..63847	+	363	WP_032694616.1	arsenite efflux transporter metallochaperone ArsD	-
FDZ05_RS29990	63864..65615	+	1752	WP_032694617.1	arsenical pump-driving ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..110998	110998
	-	inside	IScluster/Tn	-	-	47210..62776	15566

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (46-146)

Antitoxin

• Vibrio phage ICP1, Orf50 (10-86)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17678.45 Da
• Isoelectric Point: 8.9130

>T125092 WP_032694614.1 NZ_CP040186:c60427-59945 [Raoultella planticola]
MGMRAPESLTAEHNIAEFCCQEPALNEWLKKKALRNHSTGISRVYVVCAENSNRVIGYYCLSSGSVHRNTVPGAYRRNAP
EVIPVIVLGRLAIDQAWSGNGLGAALLKDAIYRTQNIAFQVGVRALAVHALNEEVKCFYTRFGFEPSIVNTLTLLFPIKV

Nucleotide

• Length: 483 bp

>T125092 NZ_CP040186:c60427-59945 [Raoultella planticola]
ATGGGAATGAGAGCGCCTGAATCCCTGACGGCTGAACATAACATCGCTGAGTTCTGCTGCCAGGAGCCTGCCCTTAATGA
GTGGTTAAAGAAGAAGGCTCTCAGAAACCACAGTACCGGCATTTCCCGTGTCTATGTCGTCTGCGCAGAAAACTCGAACC
GGGTCATCGGTTATTATTGTTTATCTTCGGGTAGCGTCCACCGTAACACAGTGCCTGGGGCCTATAGACGCAACGCGCCT
GAAGTCATTCCGGTCATTGTCCTGGGACGGCTTGCCATCGACCAGGCCTGGTCCGGGAATGGGCTTGGTGCCGCACTTCT
TAAGGATGCTATATACCGTACGCAAAACATTGCCTTTCAGGTCGGGGTGAGAGCACTGGCCGTGCATGCGCTGAATGAAG
AAGTTAAATGTTTTTACACCCGTTTTGGCTTTGAGCCTTCCATAGTCAACACGCTGACATTGTTATTCCCGATAAAAGTC
TGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10704.23 Da
• Isoelectric Point: 4.5271

>AT125092 WP_004902250.1 NZ_CP040186:c60696-60418 [Raoultella planticola]
MKPDVREAPINIRAKTSQRDLIDMAASLVSKSRTDFMLEVACREAQDILLDQRLFLLNDNQFEAFIEELDAPITPERQAR
IDNLMNRKSPWE

Nucleotide

• Length: 279 bp

>AT125092 NZ_CP040186:c60696-60418 [Raoultella planticola]
ATGAAACCAGACGTTCGTGAAGCTCCGATCAATATCCGGGCCAAAACATCACAGCGTGACCTGATCGACATGGCCGCAAG
CCTGGTCTCAAAATCACGGACTGATTTTATGCTTGAGGTTGCCTGCAGGGAAGCACAGGACATTCTACTTGATCAACGAC
TGTTCCTCCTCAATGACAATCAGTTTGAGGCGTTTATTGAAGAGCTTGACGCCCCAATAACGCCGGAGCGCCAGGCGAGA
ATCGATAACCTGATGAACCGCAAATCACCATGGGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A071LPN3