Detailed information of TA system TA125088

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 3535571..3536202
• Replicon: chromosome
• Accession: NZ_CP040183
• Organism: Raoultella planticola strain Rp_CZ180511

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: FDZ05_RS16820
• Protein ID: WP_180888584.1
• Coordinates: 3536026..3536202 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A2X1QFN9
• Locus tag: FDZ05_RS16815
• Protein ID: WP_012542176.1
• Coordinates: 3535571..3535978 (-)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FDZ05_RS16775	3531551..3532096	-	546	WP_152308856.1	terminase small subunit	-
FDZ05_RS16780	3532390..3532818	-	429	WP_132467599.1	hypothetical protein	-
FDZ05_RS16785	3532821..3533111	-	291	WP_132467600.1	hypothetical protein	-
FDZ05_RS16790	3533287..3533763	+	477	WP_165926674.1	retroviral-like aspartic protease family protein	-
FDZ05_RS16795	3533766..3534119	-	354	WP_132467602.1	hypothetical protein	-
FDZ05_RS16800	3534162..3534638	-	477	WP_180889131.1	DUF2514 domain-containing protein	-
FDZ05_RS16805	3534671..3535201	-	531	WP_180888583.1	lysozyme	-
FDZ05_RS16810	3535179..3535415	-	237	WP_004111739.1	class II holin family protein	-
FDZ05_RS16815	3535571..3535978	-	408	WP_012542176.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
FDZ05_RS16820	3536026..3536202	-	177	WP_180888584.1	type II toxin-antitoxin system HicA family toxin	Toxin
FDZ05_RS16825	3536565..3537722	+	1158	WP_020803203.1	DUF262 domain-containing protein	-
FDZ05_RS16830	3537724..3538413	+	690	WP_012542179.1	hypothetical protein	-
FDZ05_RS16835	3538379..3539422	-	1044	WP_012542180.1	DNA cytosine methyltransferase	-
FDZ05_RS16840	3539512..3539856	-	345	WP_180888585.1	antitermination protein	-
FDZ05_RS16845	3539869..3540912	-	1044	WP_180888586.1	DUF968 domain-containing protein	-
FDZ05_RS16850	3540912..3541115	-	204	WP_180888587.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3499669..3552893	53224

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-61)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6704.89 Da
• Isoelectric Point: 10.8173

>T125088 WP_180888584.1 NZ_CP040183:c3536202-3536026 [Raoultella planticola]
VKQSELRRWLAAQEAEFKDGTNHLKIYLNGKQTVMPRHPGKEIPEPLRKAILKQLGIK

Nucleotide

• Length: 177 bp

>T125088 NZ_CP040183:c3536202-3536026 [Raoultella planticola]
GTGAAGCAAAGCGAGTTGAGGCGTTGGCTTGCAGCTCAAGAGGCAGAATTTAAAGATGGTACTAACCACTTGAAAATCTA
TCTCAACGGCAAGCAAACGGTAATGCCGAGACATCCGGGGAAGGAAATACCGGAACCGCTGAGGAAGGCAATTCTTAAGC
AACTCGGCATCAAATAA

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 14977.99 Da
• Isoelectric Point: 4.4277

>AT125088 WP_012542176.1 NZ_CP040183:c3535978-3535571 [Raoultella planticola]
MRYPVIFEHDETGWAVFFPDIPEAMTGGETREEALEMAQDALVTAFDFYFDDRREIPAPSAEGDAFVEVPASVAAKVLLL
NRLVSTNTSNADLARMINTRPQEVQRIVSLGHSTKIDTIQKALSALGQKMEIVVH

Nucleotide

• Length: 408 bp

>AT125088 NZ_CP040183:c3535978-3535571 [Raoultella planticola]
ATGCGATACCCGGTAATTTTTGAGCATGACGAAACCGGCTGGGCGGTATTCTTTCCGGATATCCCAGAGGCAATGACGGG
AGGAGAGACCAGGGAGGAGGCGTTAGAAATGGCGCAAGATGCCCTGGTGACGGCGTTTGATTTTTACTTTGACGACCGCC
GGGAGATTCCCGCTCCATCAGCAGAAGGTGATGCGTTTGTTGAGGTTCCGGCCAGCGTGGCAGCTAAAGTATTGCTGTTA
AACCGTCTGGTCAGCACCAACACCAGCAATGCTGATCTCGCCCGCATGATTAACACGCGCCCACAGGAAGTGCAGCGCAT
CGTGTCTCTTGGTCACAGCACCAAAATCGATACGATCCAAAAAGCGCTATCAGCGTTGGGGCAAAAGATGGAAATTGTCG
TCCACTAA

Structures

Antitoxin

• AlphaFold DB: A0A2X1QFN9