Detailed information of TA system TA125062

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1854660..1855417
• Replicon: chromosome
• Accession: NZ_CP040182
• Organism: Serratia fonticola strain MS5

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: FD644_RS08430
• Protein ID: WP_081367093.1
• Coordinates: 1854660..1855142 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A448SFY1
• Locus tag: FD644_RS08435
• Protein ID: WP_071683113.1
• Coordinates: 1855133..1855417 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FD644_RS08405	1850580..1851317	+	738	WP_059200166.1	5-oxoprolinase subunit PxpA	-
FD644_RS08410	1851384..1852115	+	732	WP_021805279.1	DUF969 domain-containing protein	-
FD644_RS08415	1852112..1853119	+	1008	WP_071683109.1	DUF979 domain-containing protein	-
FD644_RS08420	1853128..1853772	+	645	WP_021805277.1	pyroglutamyl-peptidase I	-
FD644_RS08425	1853845..1854636	+	792	WP_071683111.1	endonuclease VIII	-
FD644_RS08430	1854660..1855142	-	483	WP_081367093.1	GNAT family N-acetyltransferase	Toxin
FD644_RS08435	1855133..1855417	-	285	WP_071683113.1	DUF1778 domain-containing protein	Antitoxin
FD644_RS08440	1855739..1857028	-	1290	WP_021805275.1	citrate synthase	-
FD644_RS08445	1857675..1858064	+	390	WP_024483149.1	succinate dehydrogenase cytochrome b556 subunit	-
FD644_RS08450	1858058..1858405	+	348	WP_021805273.1	succinate dehydrogenase membrane anchor subunit	-
FD644_RS08455	1858406..1860172	+	1767	WP_059200169.1	succinate dehydrogenase flavoprotein subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (25-148)

Antitoxin

• Vibrio phage ICP1, Orf50 (5-93)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 18015.91 Da
• Isoelectric Point: 7.7995

>T125062 WP_081367093.1 NZ_CP040182:c1855142-1854660 [Serratia fonticola]
MEIIIIAPELLTEEHILQPFDCGNEVLSDWLRRRAMRNQLLNASRTFVICLEGTKRVVGYYSIATGSVSHADLGRSLRQN
MPDPVPIVLLGRLAVDVCTQGHRFGKWLLNDAVRRVANLADQVGIKAIMVHAIDDQARAFYEYFGFVQSPIAANTLFYKI

Nucleotide

• Length: 483 bp

>T125062 NZ_CP040182:c1855142-1854660 [Serratia fonticola]
ATGGAAATAATCATTATCGCGCCGGAACTGCTGACAGAAGAGCATATTCTCCAACCATTCGACTGCGGAAATGAGGTGTT
GAGCGACTGGTTACGCCGTCGAGCCATGAGAAACCAACTCCTGAATGCCTCCCGCACTTTCGTTATTTGTCTGGAAGGCA
CCAAGCGTGTTGTGGGTTATTACTCCATTGCAACCGGTTCCGTCAGTCACGCCGATTTAGGCCGTAGCCTTCGCCAAAAC
ATGCCAGATCCTGTTCCCATCGTCCTGCTGGGACGTCTGGCCGTTGATGTATGTACCCAAGGGCATCGCTTTGGCAAGTG
GCTGCTTAATGATGCTGTAAGGCGTGTTGCCAATCTGGCAGACCAAGTGGGTATTAAAGCCATCATGGTGCACGCGATAG
ATGATCAAGCGAGAGCATTCTATGAGTACTTCGGCTTCGTCCAGTCTCCCATCGCTGCAAACACACTCTTCTACAAGATT
TGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 11066.66 Da
• Isoelectric Point: 8.5275

>AT125062 WP_071683113.1 NZ_CP040182:c1855417-1855133 [Serratia fonticola]
MQKTVRKPTRDKQINVRATEEERAVIDYAASLVNKNRTDFIIEKAVHEAQNIILDQRVFVLDDARYQAFIEQLEAPVQNV
EGRKRLMDVKPEWK

Nucleotide

• Length: 285 bp

>AT125062 NZ_CP040182:c1855417-1855133 [Serratia fonticola]
ATGCAGAAAACCGTTCGTAAACCGACTCGTGACAAACAGATTAACGTCCGGGCGACCGAAGAAGAGCGTGCAGTCATCGA
CTATGCTGCGAGTCTGGTCAATAAGAACCGGACCGACTTCATCATTGAGAAGGCGGTTCATGAAGCACAGAACATCATCC
TGGATCAGCGGGTATTCGTGCTTGATGATGCACGCTATCAGGCATTCATTGAACAGCTTGAAGCTCCTGTGCAAAACGTC
GAAGGGCGCAAGCGCTTAATGGATGTGAAGCCTGAATGGAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A448SFY1