Detailed information of TA system TA125034

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 2388218..2389940
• Replicon: chromosome
• Accession: NZ_CP040172
• Organism: Vibrio cholerae strain VC1374

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: D0FZT9
• Locus tag: FDZ90_RS11645
• Protein ID: WP_000356587.1
• Coordinates: 2388218..2389156 (-)
• Length: 313 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: A0A934MVJ4
• Locus tag: FDZ90_RS11650
• Protein ID: WP_000095577.1
• Coordinates: 2389149..2389940 (-)
• Length: 264 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FDZ90_RS11625	2384159..2384671	-	513	WP_001166556.1	S26 family signal peptidase	-
FDZ90_RS11630	2384655..2385002	-	348	WP_001044650.1	hypothetical protein	-
FDZ90_RS11635	2384995..2387394	-	2400	WP_000648041.1	type IV secretion system protein TraC	-
FDZ90_RS11640	2387394..2388086	-	693	WP_001228924.1	DsbC family protein	-
FDZ90_RS11645	2388218..2389156	-	939	WP_000356587.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
FDZ90_RS11650	2389149..2389940	-	792	WP_000095577.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
FDZ90_RS11655	2390161..2390547	-	387	WP_156843795.1	hypothetical protein	-
FDZ90_RS11660	2390544..2391116	-	573	WP_139057167.1	type IV conjugative transfer system lipoprotein TraV	-
FDZ90_RS11665	2391191..2392480	-	1290	WP_001883765.1	TraB/VirB10 family protein	-
FDZ90_RS11670	2392483..2393379	-	897	WP_032467354.1	type-F conjugative transfer system secretin TraK	-
FDZ90_RS11675	2393363..2393989	-	627	WP_000667169.1	hypothetical protein	-
FDZ90_RS11680	2393986..2394267	-	282	WP_000433889.1	type IV conjugative transfer system protein TraL	-
FDZ90_RS11685	2394448..2394771	+	324	WP_000118545.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	sul2 / aph(3'')-Ib / aph(6)-Id / floR / dfrA18	-	2302852..2450894	148042

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator, AbiEi 3 antitoxin, Type IV TA system (99-250)

Sequences

Toxin

Protein

• Length: 313 a.a.
• Molecular weight: 35548.82 Da
• Isoelectric Point: 4.9081

>T125034 WP_000356587.1 NZ_CP040172:c2389156-2388218 [Vibrio cholerae]
MDKDSPYYKQVSLLIRMLPVVATETVFALKGGTAINLFVRDFPRLSVDIDLAYLPLEPRDEALINVRAALQRITDRINTQ
PDIRAAFQDNKADELRIVVSSPVATIKIEVSPVARGTLHSAEIMPVQESVEDEFGYAEIQVVSLPDLYGGKLCAAMDRQH
PRDLFDVRMLLGSEGISREILVGFLTYTLSHPRPINEVMSPNWQPLNEKFQAEFDGMTFEKVECEDLASVRPMMLIELQK
HFTERDHAFLMSFKRGQPDWALFDYPNAADLPAIRWKLQNINKLAKNQAKHQEQLDKLKQVLDDWLVNANAE

Nucleotide

• Length: 939 bp

>T125034 NZ_CP040172:c2389156-2388218 [Vibrio cholerae]
ATGGATAAAGATAGCCCATATTACAAACAGGTTTCCTTGCTCATAAGAATGCTTCCTGTGGTAGCAACAGAGACGGTTTT
TGCACTTAAAGGTGGCACCGCCATTAATTTATTTGTGAGAGATTTTCCTCGGTTATCTGTAGATATTGATCTTGCTTATC
TTCCACTTGAACCAAGAGATGAGGCTTTGATTAATGTCAGGGCTGCATTGCAGCGCATTACAGACAGAATCAATACTCAA
CCAGATATCAGAGCGGCATTTCAGGATAATAAAGCTGATGAACTGAGAATAGTTGTATCAAGTCCGGTTGCGACGATCAA
AATTGAAGTGTCGCCCGTCGCCAGAGGTACATTGCATAGTGCAGAAATAATGCCAGTTCAAGAGTCTGTTGAAGACGAGT
TTGGTTATGCTGAGATTCAGGTAGTCAGTCTTCCCGATCTGTATGGTGGGAAATTGTGTGCTGCAATGGATCGCCAACAT
CCTCGCGACTTATTTGATGTGCGTATGTTACTTGGCAGTGAAGGGATTTCGAGAGAGATTTTGGTGGGTTTTTTAACCTA
TACATTAAGTCACCCTCGGCCTATTAATGAAGTCATGTCGCCAAACTGGCAGCCTCTGAATGAGAAATTTCAGGCAGAAT
TTGACGGAATGACTTTTGAAAAAGTTGAATGCGAAGACTTAGCCTCTGTTAGGCCCATGATGTTAATTGAACTGCAAAAA
CATTTCACAGAAAGGGATCATGCTTTCCTTATGTCATTCAAAAGAGGGCAACCTGACTGGGCATTGTTTGACTATCCTAA
TGCAGCAGACTTGCCAGCGATTCGTTGGAAATTGCAGAACATTAACAAATTGGCAAAGAATCAAGCAAAACACCAAGAGC
AATTAGATAAATTGAAGCAAGTGCTTGATGATTGGCTTGTTAACGCAAACGCCGAGTAA

Antitoxin

Protein

• Length: 264 a.a.
• Molecular weight: 30109.49 Da
• Isoelectric Point: 9.5097

>AT125034 WP_000095577.1 NZ_CP040172:c2389940-2389149 [Vibrio cholerae]
MSSKINWLVAHTSPGALVLQQWLTENGVSYSLAQKYAQNGWLKKLSSGVYYRPNAQGDIKPTWVDAIQALDVQLGVPVHL
AGLSSLTHQGLSHYLQLNKEQVWICVKNKSSLPKWFREFPYQNWFYCGNHKLEVNPEKDLKRITVKEKELTVSCAELAAY
EVVDAIGKLISFEHVAELFQGLVNLSPRKVQDILERSSSVQANRIFLFLGRYYDHQWVNRIDETRIKLGAGKRQVVEKGR
FDERYQITVPEILSVKKGEQHNG

Nucleotide

• Length: 792 bp

>AT125034 NZ_CP040172:c2389940-2389149 [Vibrio cholerae]
ATGTCATCTAAAATAAACTGGCTTGTTGCTCATACTTCTCCTGGTGCGCTAGTACTTCAGCAATGGCTGACTGAAAACGG
CGTGAGCTACTCGTTGGCTCAAAAGTACGCGCAGAACGGTTGGCTGAAGAAGCTTAGTTCTGGTGTGTACTATCGTCCAA
ATGCGCAGGGCGATATAAAGCCAACTTGGGTTGATGCCATTCAAGCATTGGATGTGCAATTGGGCGTTCCAGTTCATTTG
GCTGGATTGAGCAGCTTAACTCACCAAGGACTGAGTCACTATTTACAGCTGAACAAAGAGCAAGTTTGGATTTGCGTTAA
AAACAAGTCGTCCTTACCGAAATGGTTTCGTGAATTCCCTTATCAGAATTGGTTTTACTGCGGAAACCATAAGCTTGAAG
TGAATCCCGAGAAAGATTTGAAAAGGATCACGGTTAAAGAGAAAGAGCTCACTGTCAGCTGTGCAGAACTTGCTGCCTAT
GAAGTGGTAGATGCGATTGGAAAGCTGATTTCATTTGAGCATGTCGCAGAATTATTTCAGGGTTTAGTCAATCTTAGCCC
TAGAAAAGTACAAGATATTCTTGAACGAAGCAGCTCTGTTCAGGCAAACCGAATATTTCTATTTCTGGGTCGATACTACG
ATCACCAGTGGGTCAATCGCATAGATGAAACAAGAATTAAATTGGGGGCAGGAAAGCGGCAGGTTGTCGAAAAAGGACGT
TTTGATGAGCGATATCAAATCACAGTGCCAGAGATATTAAGCGTCAAAAAAGGTGAACAACATAATGGATAA

Structures

Toxin

• AlphaFold DB: A0A1U9VZ44