Detailed information of TA system TA125025

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PfiT-PfiA/ParE(toxin)
• Location: 4882328..4882936
• Replicon: chromosome
• Accession: NZ_CP040127
• Organism: Pseudomonas aeruginosa strain PA298

Toxin (Protein)

• Gene name: PfiT
• Uniprot ID: -
• Locus tag: E6P00_RS25670
• Protein ID: WP_016263850.1
• Coordinates: 4882328..4882675 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: PfiA
• Uniprot ID: A0A0B0C355
• Locus tag: E6P00_RS25675
• Protein ID: WP_003114155.1
• Coordinates: 4882685..4882936 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E6P00_RS25640	4877687..4877959	+	273	WP_019725834.1	cysteine-rich CWC family protein	-
E6P00_RS25645	4877959..4878651	+	693	WP_003098362.1	16S rRNA pseudouridine(516) synthase	-
E6P00_RS25650	4878787..4879830	+	1044	WP_003098363.1	L,D-transpeptidase	-
E6P00_RS25655	4879910..4880647	+	738	WP_003114158.1	murein L,D-transpeptidase catalytic domain family protein	-
E6P00_RS25660	4881099..4882001	+	903	WP_003123042.1	(R)-3-hydroxydecanoyl-ACP:CoA transacylase	-
E6P00_RS25670	4882328..4882675	-	348	WP_016263850.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E6P00_RS25675	4882685..4882936	-	252	WP_003114155.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
E6P00_RS25680	4883150..4884133	-	984	WP_019725833.1	tyrosine-type recombinase/integrase	-
E6P00_RS25685	4884133..4885425	-	1293	WP_003115206.1	hypothetical protein	-
E6P00_RS25690	4885684..4886946	-	1263	WP_019725831.1	hypothetical protein	-
E6P00_RS25695	4886948..4887298	-	351	WP_019725830.1	DUF2523 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	catB7	-	4882328..4904471	22143

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-100)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13046.85 Da
• Isoelectric Point: 4.4212

>T125025 WP_016263850.1 NZ_CP040127:c4882675-4882328 [Pseudomonas aeruginosa]
MSPVVIRFTDTAEQSIEDQVHHLVPFQGEQAAFQSVLSLLDEIEEKISLAPKGYPVSQQASLLGVLSYRELNTGPYRVFY
EFHEEQGEVAVILVLRQKQSVEQQLIRYCLVGPIE

Nucleotide

• Length: 348 bp

>T125025 NZ_CP040127:c4882675-4882328 [Pseudomonas aeruginosa]
ATGTCCCCGGTCGTCATTCGTTTTACTGATACCGCAGAGCAAAGCATCGAAGACCAAGTCCACCACTTGGTTCCATTCCA
AGGTGAACAGGCTGCATTCCAGTCAGTACTGAGCCTTTTGGATGAGATTGAAGAGAAGATTTCACTTGCACCTAAAGGTT
ACCCAGTCAGCCAGCAGGCGAGTCTTCTGGGGGTGCTGAGCTATCGCGAGCTTAATACCGGCCCCTATCGTGTTTTTTAC
GAATTCCACGAAGAGCAAGGCGAGGTGGCAGTGATCTTGGTTTTGCGACAGAAGCAGAGCGTTGAGCAGCAATTGATCCG
CTACTGCTTGGTGGGGCCAATCGAGTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9442.79 Da
• Isoelectric Point: 9.4808

>AT125025 WP_003114155.1 NZ_CP040127:c4882936-4882685 [Pseudomonas aeruginosa]
MRVETISYLKRHAADLDLSEPMVVTQNGVPAYVVESYAERKQRDEAIALVKLLAIGSRQYAEGKHRSVDDLKARLSRRFA
QPE

Nucleotide

• Length: 252 bp

>AT125025 NZ_CP040127:c4882936-4882685 [Pseudomonas aeruginosa]
ATGCGAGTCGAGACAATTAGTTATTTGAAACGTCATGCGGCTGACTTGGATTTATCCGAGCCAATGGTCGTCACGCAGAA
CGGTGTTCCTGCCTATGTGGTTGAGTCATATGCTGAGCGGAAGCAGCGCGATGAAGCAATTGCGCTGGTGAAGTTGCTTG
CGATTGGCTCCCGCCAGTACGCAGAAGGCAAGCATCGCTCTGTTGATGATTTGAAAGCTCGCCTTTCCAGGAGGTTCGCT
CAGCCAGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0B0C355