Detailed information of TA system TA125019

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 149029..149534
• Replicon: chromosome
• Accession: NZ_CP040127
• Organism: Pseudomonas aeruginosa strain PA298

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: E6P00_RS03065
• Protein ID: WP_003083773.1
• Coordinates: 149029..149310 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1C7BDS9
• Locus tag: E6P00_RS03070
• Protein ID: WP_003083775.1
• Coordinates: 149307..149534 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E6P00_RS03040	144280..145629	+	1350	WP_003142411.1	C4-dicarboxylate transporter DctA	-
E6P00_RS03045	145678..146364	+	687	WP_003083762.1	FadR family transcriptional regulator	-
E6P00_RS03050	146465..147199	+	735	WP_003117208.1	GntR family transcriptional regulator	-
E6P00_RS03055	147403..147789	+	387	WP_014602345.1	aegerolysin family protein	-
E6P00_RS03060	147821..148729	-	909	WP_003083769.1	LysR family transcriptional regulator	-
E6P00_RS03065	149029..149310	-	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
E6P00_RS03070	149307..149534	-	228	WP_003083775.1	CopG family ribbon-helix-helix protein	Antitoxin
E6P00_RS03075	149710..150330	-	621	WP_009314940.1	hypothetical protein	-
E6P00_RS03080	150431..150931	+	501	WP_003112629.1	LEA type 2 family protein	-
E6P00_RS03085	151004..151345	+	342	WP_003101229.1	alkylphosphonate utilization protein	-
E6P00_RS03090	151427..152854	-	1428	WP_003083784.1	GABA permease	-
E6P00_RS03095	153023..154516	-	1494	WP_003083788.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T125019 WP_003083773.1 NZ_CP040127:c149310-149029 [Pseudomonas aeruginosa]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T125019 NZ_CP040127:c149310-149029 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8556.58 Da
• Isoelectric Point: 5.5740

>AT125019 WP_003083775.1 NZ_CP040127:c149534-149307 [Pseudomonas aeruginosa]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHTEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT125019 NZ_CP040127:c149534-149307 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Toxin

• PDB: 6XRW

Antitoxin

• AlphaFold DB: A0A1C7BDS9