Detailed information of TA system TA125018

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 3855..4435
• Replicon: plasmid pLSH-KPN148-3
• Accession: NZ_CP040125
• Organism: Klebsiella pneumoniae strain LSH-KPN148

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A8J3DTL7
• Locus tag: FDY04_RS28165
• Protein ID: WP_071177730.1
• Coordinates: 3855..4169 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2X1PRM1
• Locus tag: FDY04_RS28170
• Protein ID: WP_000093040.1
• Coordinates: 4157..4435 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FDY04_RS28145	1763..2494	+	732	WP_071177728.1	replication-relaxation family protein	-
FDY04_RS28150	2501..3031	+	531	WP_071177729.1	hypothetical protein	-
FDY04_RS28155	3058..3237	-	180	WP_000165970.1	Rop family plasmid primer RNA-binding protein	-
FDY04_RS28160	3263..3691	-	429	WP_001140599.1	hypothetical protein	-
FDY04_RS28165	3855..4169	-	315	WP_071177730.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FDY04_RS28170	4157..4435	-	279	WP_000093040.1	CopG family ribbon-helix-helix protein	Antitoxin
FDY04_RS28175	4769..4975	-	207	Protein_7	TonB family protein	-
FDY04_RS28180	4972..5343	-	372	WP_001237044.1	cell envelope integrity protein TolA	-
FDY04_RS28185	5617..5862	-	246	WP_032440458.1	hypothetical protein	-
FDY04_RS28190	6189..7874	+	1686	WP_032440457.1	cloacin	-
FDY04_RS28195	7884..8141	+	258	WP_032440455.1	cloacin	-
FDY04_RS28200	8226..8375	+	150	WP_032440454.1	colicin release lysis protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..10060	10060

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11805.73 Da
• Isoelectric Point: 9.8324

>T125018 WP_071177730.1 NZ_CP040125:c4169-3855 [Klebsiella pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKTLPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDR
EMDQIVIVTVRHQKESGYPGADSL

Nucleotide

• Length: 315 bp

>T125018 NZ_CP040125:c4169-3855 [Klebsiella pneumoniae]
ATGCCACAGGTAACGATTTCCGCGCTGGCACAGCGCGATTTACAACGCCTTCAGGACTTTCTCAAAACCAAAAATCGGCT
GGCAGCCAGAAAAGGAGGTGAGGTGATCGTCCGGGCTATCCAGCAACTGAAAACATTGCCAGACATTGGCCGCCCGGTGC
CGTTTCTGCCGCTGGAATATAAGGAACTGGTGATCGGGTTTGGCGACAGTGGCTATGTGATGCTCTACCGCCACGACCGG
GAAATGGACCAGATTGTGATCGTCACCGTCAGGCATCAGAAAGAATCCGGGTATCCGGGGGCAGACAGCCTCTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10709.73 Da
• Isoelectric Point: 4.7561

>AT125018 WP_000093040.1 NZ_CP040125:c4435-4157 [Klebsiella pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT125018 NZ_CP040125:c4435-4157 [Klebsiella pneumoniae]
ATGTCATCAGCAACATCCATCAGGCTCGATGAAGAACTTAAGGACCGGCTGAAAACGCTGGCCGATGACCGGCACCGCTC
AGCGCACGCGCTAATGCTGGAGGCAATCACCGAATACATCGACCGGGAAGAGAAGCGCAGCCAGTACCTGCGGGACGGAC
AGGCCGCATGGCAGCACTATCAGGAAACGGGTCTGCACCTTACTGCGGAAGAAGCGGAAGCCTGGATAAGTACCTGGGGA
ACTGAGAACGAGCAGGATGCTCCGCCATGCCACAGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2X1PRM1